A kind of cultivation method of tetraploid non-heading Chinese cabbage
A technology of head cabbage and its cultivation method, which is applied in the field of genetic breeding and can solve the problems of low water solubility and the like
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Embodiment 1
[0025] Step 1: Select 216 plump seeds of non-heading Chinese cabbage harvested in the same year.
[0026] Step 2, the seeds are subjected to germination treatment, and the seeds are dialed into plug trays after being white. When the cotyledons of the seedlings are fully expanded, the mixed aqueous solution of amisulfuryl (concentration of amisulfuryl is 0.06mmol·L -1 , the concentration of Tween 80 is 0.02% v / v, and the concentration of Silwet L-77 is 0.02% v / v) in the morning and evening at the growth point of the seedlings, and the treatment is done 5 times.
[0027] Step 3, after the adult plants are grown, the tetraploid plants are first screened according to the length and width of the 5th to 10th true leaves, the degree of twisting of the leaves, the thickness of the main stem, and the plant height. Through the identification of stomata in anatomy, the tetraploid variation primary plants were determined, and the variation rate reached 4.63%.
[0028] Step 4: Enter the ...
Embodiment 2
[0043] Step 1: Select 212 plump seeds of non-heading Chinese cabbage harvested in the same year.
[0044] Step 2, the seeds are subjected to germination treatment, and the seeds are dialed into plug trays after being white. When the cotyledons of the seedlings are fully unfolded, the mixed aqueous solution of amisulfuryl (the concentration of amisulfuryl is 0.08mmol·L -1 , the concentration of Tween 80 is 0.02% v / v, and the concentration of Silwet L-77 is 0.02% v / v) in the morning and evening at the growth point of the seedlings, and the treatment is done 5 times.
[0045] Step 3, after the adult plants are grown, the tetraploid plants are first screened according to the length and width of the 5th to 10th true leaves, the degree of twisting of the leaves, the thickness of the main stem, and the plant height. Then through the identification of stomata in anatomy, the primary plants for tetraploid variation were determined, and the variation rate reached 9.91%.
[0046] Step ...
Embodiment 3
[0056] Step 1: Select 199 plump seeds of non-heading Chinese cabbage harvested in the same year.
[0057] Step 2, the seeds are subjected to germination treatment, and the seeds are dialed into plug trays after being white. When the cotyledons of the seedlings are fully unfolded, the mixed aqueous solution of amisulfuryl (the concentration of amisulfuryl is 0.1mmol·L -1 , the concentration of Tween 80 is 0.02% v / v, and the concentration of Silwet L-77 is 0.02% v / v) in the morning and evening at the growth point of the seedlings, and the treatment is done 5 times.
[0058] Step 3, after the adult plants are grown, the tetraploid plants are first screened according to the length and width of the 5th to 10th true leaves, the degree of twisting of the leaves, the thickness of the main stem, and the plant height. Through the identification of stomata in anatomy, the tetraploid variation primary plants were determined, and the variation rate reached 8.04%.
[0059] Step 4: Enterin...
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