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Biological enzyme method for extracting N-acetylglucosamine and astaxanthin from crawfish shells

An acetamido, biological enzymatic technology, applied in the biological field, can solve the problems of restricting the development of the biological sugar industry, dangerous operation, expensive equipment, etc., and achieve the effects of shortening the production cycle, high safety and low cost

Inactive Publication Date: 2018-09-21
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned technologies are relatively mature, there are disadvantages of expensive equipment and dangerous operation, which limit the development of biological sugar industry

Method used

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  • Biological enzyme method for extracting N-acetylglucosamine and astaxanthin from crawfish shells
  • Biological enzyme method for extracting N-acetylglucosamine and astaxanthin from crawfish shells
  • Biological enzyme method for extracting N-acetylglucosamine and astaxanthin from crawfish shells

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Experimental program
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Embodiment 1

[0030] A method for extracting N-acetylglucosamine and astaxanthin from crayfish shells by a biological enzyme method, comprising the following steps:

[0031] Step 1, pretreatment of crayfish shells

[0032] 50g crayfish shells were ultrasonically cleaned 3 times, once for 30 minutes to remove most of the particle impurities, boiled to remove edible oil, then dried, crushed (50 mesh) to obtain crayfish shell powder;

[0033] Step 2, preparation of crude enzyme solution

[0034] Take chitinase-producing microorganisms as the test bacteria, inoculate the test bacteria on the seed medium, and cultivate the seed liquid under the conditions of 37°C, 200 rpm, and initial pH 7, and then transfer the seed liquid to the fermentation culture After fermenting in the medium at 30°C, 200 rpm, initial pH of 7, and aeration of 1vvm, the crude enzyme liquid was purified;

[0035] Step 3, aseptic enzymatic hydrolysis of crayfish shells

[0036] Take 10g of crayfish shrimp shell powder and ...

Embodiment 2

[0039] A method for extracting N-acetylglucosamine and astaxanthin from crayfish shells by a biological enzyme method, comprising the following steps:

[0040] Step 1, pretreatment of crayfish shells

[0041] 500g crayfish shells were ultrasonically cleaned for 3 times, once for 40 minutes to remove most of the particle impurities, boiled to remove edible oil, then dried, crushed (50 mesh) to obtain crayfish shell powder;

[0042] Step 2, preparation of crude enzyme solution

[0043] Take chitinase-producing microorganisms as the test bacteria, inoculate the test bacteria on the seed medium, and cultivate the seed liquid under the conditions of 37°C, 200 rpm, and initial pH 7, and then transfer the seed liquid to the fermentation culture After fermentation in the medium at 30°C, 200 rpm, initial pH 6.8, and air flow 1vvm, the crude enzyme liquid was purified;

[0044] Step 3, aseptic enzymatic hydrolysis of crayfish shells

[0045] Take 10g of crayfish shrimp shell powder a...

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Abstract

The invention discloses a biological enzyme method for extracting N-acetylglucosamine and astaxanthin from crawfish shells. The method comprises the following steps that pretreatment is carried out onthe crawfish shells to obtain crawfish shell powder; microorganisms producing chitinase are taken as testing bacterium, the testing bacterium is inoculated to a seed culturing medium for culturing soas to obtain seed liquid, and after the seed liquid is transferred to a fermentation culturing medium for fermentation, coarse pure enzyme liquid is obtained through purification; the crawfish shellpowder is placed to a reaction still, the coarse pure enzyme liquid and organic reagent are successively added, under the circumstance of keeping out of the sun, the mixture is stirred and heated synchronously at the constant temperature to 20-37 DEG C, and enzymatic hydrolysate is obtained; after static layering is performed on the enzymatic hydrolysate, supernatant is poured out, under the circumstance of keeping out of the sun, the organic reagent and crawfish oil containing rich astaxanthin are recovered, subnatant is centrifuged, and after adsorption and elution of adsorbent resin are conducted, eluant is subjected to low-temperature concentration, ethanol washing, crystallization and drying to obtain the acetylglucosamine. The method takes waste biomass as raw materials, two substances are extracted, the producing period is shortened, and the product quality is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for extracting N-acetylglucosamine and astaxanthin from crayfish shells by a biological enzyme method. Background technique [0002] N-acetyl-D-glucosamine is the basic unit of many important polysaccharides in biological cells, especially in the exoskeleton of marine crustaceans. N-acetyl-D-glucosamine and its derivatives are widely used. In clinical medicine, it is currently the drug for treating rheumatism and rheumatoid arthritis. In addition, it also has the function of enhancing the human immune system. By inhibiting cancer The overgrowth of cells or fibroblasts can inhibit and treat cancer and malignant tumors. In the food industry, it can also be used as food antioxidants, food additives for infants and sweeteners for diabetics. [0003] Astaxanthin, also known as lobster shell pigment, has two β-ionone rings and 11 conjugated double bonds in its molecular structur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12P19/26
CPCC12P19/26C12P23/00
Inventor 陈可泉张阿磊魏国光王昕许晟欧阳平凯
Owner NANJING UNIV OF TECH