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A kind of chlorocyclopentenone compound and its preparation and application

A technology for chlorocyclopentenone and cyclopentenone is applied in the field of chlorocyclopentenone compounds and their preparation, and can solve problems such as biological and human health hazards, water body and soil pollution and the like

Active Publication Date: 2021-02-19
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, with the development of aquaculture scale and the extensive use of traditional antibiotics in the aquaculture process, multi-drug resistance of pathogenic bacteria in aquaculture animals and bacteria in the aquaculture environment has become increasingly common, and the extensive use of chemically synthesized drugs has negative effects on water bodies and soils. It has brought serious pollution, which has caused direct and potential harm to organisms and human health.

Method used

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  • A kind of chlorocyclopentenone compound and its preparation and application
  • A kind of chlorocyclopentenone compound and its preparation and application
  • A kind of chlorocyclopentenone compound and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The structure of the chlorinated cyclopentenone compound derived from the endophytic fungus of seaweed is shown in formula (I).

[0025]

[0026] The compound has the following physicochemical and spectroscopic properties:

[0027] Colorless oil; specific rotation [α] 25 D +7.5 (c 0.040, MeOH); H NMR spectrum (solvent is deuterated methanol) δ H 2.88ddd(18.6,6.3,0.9),2.30dd(18.6,1.7),5.14dd(6.3,1.5),4.97q(6.9),1.51d(6.9); C NMR spectrum (solvent is deuterated chloroform)δ C 200.1(C),44.0(CH 2 ), 68.4(CH), 175.4(C), 131.5(C), 66.3(CH), 21.8(CH 3 ); high-resolution mass spectrometry [M] + m / z 176.0236, calculated 176.0240.

Embodiment 2

[0029] The preparation method of the chlorocyclopentenone compound as shown in formula (I):

[0030] Get the Trichoderma asperellum (Trichoderma asperellum) cf44-2 bacterial classification that grows well on the plate, cut into small pieces and inoculate in the potato dextrose liquid medium, put 300 milliliters of medium in every 1 liter of Erlenmeyer flasks, totally 200 bottles, Static fermentation at room temperature for 30 days, the fermentation product was extracted three times with ethyl acetate, concentrated under reduced pressure, and a total of 83.7 grams of crude extract were obtained after concentration.

[0031] The potato glucose liquid medium consists of 500 milliliters of boiled juice containing 200 grams of potatoes per liter, 20 grams of glucose, 5 grams of peptone, 5 grams of yeast powder, and 500 milliliters of aged seawater.

[0032] Trichoderma asperellum cf44-2 (Trichoderma asperellum cf44-2) strain was preserved in China Center for Type Culture Collection...

Embodiment 3

[0037] The difference from Example 2 is that

[0038] Get the Trichoderma asperellum cf44-2 (Trichoderma asperellumcf44-2) strain that grows well on the plate, cut into small pieces and inoculate in Jerusalem artichoke glucose liquid medium, put 300 milliliters of culture medium in every liter of Erlenmeyer flask, totally 200 bottle, and fermented at room temperature for 40 days, then extracted three times with dichloromethane, concentrated under reduced pressure, and obtained 80 grams of crude extract after concentration.

[0039] The Jerusalem artichoke glucose liquid medium consists of 500 milliliters of boiled juice containing 200 grams of Jerusalem artichoke tubers per liter, 20 grams of glucose, 5 grams of peptone, 5 grams of yeast powder, and 500 milliliters of aged seawater.

[0040] The crude extract was subjected to 200-300 mesh silica gel column chromatography, followed by volume ratios of 50:1, 30:1, 15:1, 10:1, 5:1, 2:1, 1:1 to 0:1 Petroleum ether-ethanol for gra...

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Abstract

The invention relates to the field of bacteriostatic agents, in particular to a chlorocyclopentenone compound derived from a seaweed endophyte fungus, a preparation method thereof, and an application in bacteriostasis. Concrete structural formula is shown in (1), its preparation method is that seaweed endophytic fungus Trichoderma asperellum (Trichoderma asperellum) cf44-2 is inoculated in the fungal medium for fermentation culture, and the fermentation product is the formula (1 ) of the chlorocyclopentenone compounds shown. For the chlorocyclopentenone compounds obtained by the present invention, the antibacterial activity experiment shows that the diameter of the antibacterial zone of the compound at 20 micrograms / tablet can reach 8.5 mm

Description

technical field [0001] The invention relates to the field of antibacterial agents, in particular to a chlorinated cyclopentenone compound derived from seaweed endophytic fungi, a preparation method thereof and an application in antibacterial aspects. Background technique [0002] The aquaculture industry occupies an important proportion in the national economy. At present, there are various types of aquaculture, but almost all of them are threatened by diseases. The frequent occurrence of aquaculture diseases not only causes significant economic losses, but also lays hidden dangers for the quality and safety of aquatic products. According to the monitoring results of aquaculture animal diseases, there are more than 200 kinds of aquaculture diseases in recent years, of which bacterial diseases account for more than 48%. Among bacterial aquatic animal diseases, vibriosis caused by Vibrio bacteria is the most important bacterial disease. The disease has a wide range of occurre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C45/78C07C45/79C07C49/707A01N35/06A01P1/00C12N1/14C12R1/885
CPCA01N35/06C07C45/78C07C45/79C07C49/707C12N1/14C07C2601/10C12N1/145C12R2001/885
Inventor 季乃云宋银平
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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