Leuconostoc mesenteroides cjlm119 strain producing reduced amount of gas, and kimchi production method using same
A technology of CJLM119 and Leuconostoc enterococci, which is applied in Leuconostoc enteritidis strains and the production of spicy cabbage, can solve the problems of reduced food quality, damage and leakage, container expansion, etc., achieve stable circulation quality, and prevent packaging damage Effect
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Embodiment 1
[0042] Example 1: Isolation of strains with low gas production
[0043] 1-1) Strain isolation and identification
[0044] Various kinds of kimchi purchased from a large supermarket were aged at a low temperature of 5° C., and kimchi reaching a pH range of 3.8 to 4.5 was used as kimchi samples. Dilute the above-mentioned spicy cabbage sample to 10 times with 0.85% saline, and inoculate it into PES agar medium (phenylethanol sucrose agar; tryptone 5g, yeast extract 0.5g, sucrose 20g, ammonium sulfate 2g, hydrogen phosphate 1 g of dipotassium, 0.244 g of magnesium sulfate, 2.5 ml of phenylethyl alcohol, 15 g of agar / 1 L of distilled water) were placed on the plate, and spread with a spreader. Then, after culturing the above plate in a constant temperature incubator at 25° C. for 24 hours, each of the generated colonies was streaked onto another PES agar plate to separate into individual colonies.
[0045] 1-2) Screening for strains with low gas production
[0046] The bact...
Embodiment 2
[0053] Example 2: Comparing Gas Generation Capabilities
[0054] In order to compare the degree of gas production of Leuconostoc enteritidis CJLM119 screened in Example 1 with other Leuconostoc enteritidis strains, Leuconostoc enteritidis KCTC3100 and KCTC3722, which are standard strains of Leuconostoc enteritidis, were used as controls group and measure the degree of gas production. Each strain was inoculated into 10 ml of MRS broth in a test tube containing a Durham tube having a height of 30 mm, and after culturing at 25° C. for 24 hours, gas was compared by measuring the height of gas trapped in the Durham tube. degree of generation.
[0055] The degree of gas generation is expressed as "-" in the case of no gas generation, "+" in the case of gas trapped in the Durham tube at a height of 1 mm to 5 mm, and in the case of a gas trapped at a height of 6 mm to 10 mm. "++" in the case of the case, "+++" in the case of the height of 11mm to 15mm, "++++" in the case of the heig...
Embodiment 3
[0059] Example 3: Comparison of Acid Generation Capabilities
[0060] The degree of acid production of Leuconostoc enteritidis CJLM119 screened in Example 1 was compared with that of Leuconostoc enteritidis KCTC3100 and KCTC3722 as the control group. Each strain was inoculated into 10 ml of MRS broth, respectively, and in order to measure the degree of acid production, the pH after static culture at 25° C. for 24 hours was measured using a pH meter (SevenCompact / Ion S220, Mettler Toledo).
[0061] As a result, it was confirmed that the amount of acid produced by Leuconostoc enterolis CJLM119 was significantly lower than that of the control strain (Table 2).
[0062] [Table 2]
[0063]
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