A molecular marker marker Sex 1 and identification method for rapid identification of genetic sex of Portunus trituberculatus
A technology of Portunus trituberculatus and molecular markers, which is applied to biochemical equipment and methods, DNA/RNA fragments, and microbial determination/inspection, etc., can solve problems such as sex molecular markers of Portunus trituberculatus that have not yet been seen, and achieve high accuracy, The operation is simple and fast, and the effect of low sample requirements
Active Publication Date: 2019-09-03
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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However, there is no report on sex molecular markers in Portunus trituberculatus
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Embodiment 1
[0023] 1) DNA extraction
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The invention provides a molecular marker Marker Sex 1 for quickly identifying the genetic sex of Portunus trituberculatus and an identification method, and the molecular marker Marker Sex 1 has the nucleotide sequence shown in SEQ ID NO:1. The identification method comprises the following steps: extracting the genomic DNA of the muscle tissue of Portunus trituberculatus; performing a PCR amplification reaction using the extracted genomic DNA as a template; purifying and sequencing the PCR amplification product; combining the sequencing result with the molecular marker The DNA sequence is compared, and if there is a double peak, it is male, and if there is a single peak, it is female. The identification method of the present invention is simple and quick to operate, has low requirements for samples, high accuracy of identification results, and is not affected by tissue specificity and environment, and is of great importance in promoting the establishment of all-female or high-female-ratio seedling cultivation technology. effect.
Description
technical field [0001] The invention belongs to the technical field of aquatic animal biology, and in particular relates to a molecular marker Marker Sex 1 for rapidly identifying the genetic sex of Portunus trituberculatus and an identification method. Background technique [0002] Portunus trituberculatus ( Portunus trituberculatus ) belongs to Crustacea, Decapoda, Portunidae, and Portunus. It is an important fishery catch and mariculture object in my country. It has fast growth, tender meat, delicious taste, and nutrition Rich and other advantages, it is an important seafood with high economic value, and the market demand continues to increase. The economic value of Portunus trituberculatus female crabs is significantly higher than that of male crabs. Therefore, in order to improve the breeding efficiency, it is urgent to break through the breeding and identification methods of all-female or high-female ratio seedlings of Portunus trituberculatus. One of the key technolog...
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IPC IPC(8): C12Q1/6879C12N15/11
CPCC12Q1/6879
Inventor 吕建建刘萍李健高保全任宪云
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI