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SNP molecular marker related to sow leg-foot bone density

A molecular marker, bone mineral density technology, applied in recombinant DNA technology, microbial determination/test, DNA/RNA fragments, etc., can solve the problems of high price, limited application, rare livestock skeleton research, etc., to achieve high sensitivity and specificity. Good results

Active Publication Date: 2018-10-09
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that bone density is an important indicator in the study of animal bone strength, but due to the limitations of various production conditions, people's research on livestock bones is very rare
[0004] Bone mineral density, that is, the content of bone mineral per unit volume, the unit is g / cm3, common bone density measurement methods include radioabsorption method, dual-energy X-ray measurement method, quantitative computed tomography technology and axial ultrasonic transmission technology, etc. (Cao Jian, Application evaluation of bone mineral density (BMD) measurement[J], Frontiers of Medicine, 2013(2)), among which dual-energy X-ray measurement and quantitative computed tomography technology are the most widely used, but dual-energy X-ray measurement technology is the most accurate Bone density is area, affected by the geometric volume of the object, and quantitative computed tomography can measure bone density in animals more accurately, but its equipment is expensive (Liu Jun, dual-energy X-ray absorptiometry and quantitative CT to measure bone density Comparative study [D], Central South University, 2005), and the operation is very troublesome (for large livestock, it needs to be performed after anesthesia), which greatly limits its application in animal bone density research

Method used

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  • SNP molecular marker related to sow leg-foot bone density
  • SNP molecular marker related to sow leg-foot bone density
  • SNP molecular marker related to sow leg-foot bone density

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Determination of Bone Density of Sow Limbs and Hooves

[0024] (1) Bone Densitometry Instrument Number and Measurement Site

[0025] 1) Bone density measurement technology: ultrasonic bone axis transmission technology;

[0026] 2) Measurement site: the medial metatarsal bone of the hind limb of the sow

[0027] (2) SQV inspection (inspect once a day, after starting up)

[0028] 1) After the instrument is connected, turn on the computer, double-click the icon on the desktop, and open the software;

[0029] 2) In the dialog box that pops up, enter "user name": sunlight, "password": omni, and enter the bone strength interface;

[0030] 3) "Measurement type" select SQV test;

[0031] 4) After applying couplant on the probe, put the module on the probe and remove air bubbles;

[0032] 5) Click the "Measurement" button on the interface;

[0033] 6) Click the Enter key or click "Measurement" with the mouse, repeat three times;

[0034] 7) Click the "Result" bu...

Embodiment 2

[0047] Example 2 Genotyping detection

[0048] (1) Using the kit method to extract ear tissue DNA from bone density-related populations

[0049] The kit in this example uses the TIANamp Genomic DNA Kit Blood / Cell / Tissue Genomic DNA Extraction Spin Column Kit, produced by Beijing Tiangen Biochemical Technology Co., Ltd.

[0050] Specific steps are as follows:

[0051] 1) Use ophthalmic scissors to cut the ear-like tissue of the bone density-related population (this population includes Danish (Denmark) Large White and Changbai, which are conventional varieties, provided by Guangxi Yangxiang Co., Ltd.) until it becomes a paste, add 200ulGA, Shake and mix well; then put it into a 56°C water bath for overnight digestion.

[0052] 2) Add 200ul buffer GB (included with the kit) to the digested tissue sample, mix thoroughly by inversion, and place at 70°C for 10 minutes, the solution should become clear, and centrifuge briefly to remove water droplets on the tube wall.

[0053] 3) ...

Embodiment 3

[0061] Example 3 Application of the H3GA0030503 molecular marker typing method in the correlation analysis of bone density traits of pig limbs and hooves

[0062] (1) Correlation analysis between H3GA0030503 molecular marker typing results and bone density traits of limbs and hooves

[0063] The experimental pigs used for the correlation detection and analysis of genotype and bone density traits of limbs and hooves come from purebred sow groups bred by Guangxi Yangxiang Co., Ltd., including Large White pigs and Landrace pigs (conventional breeds). The DNA used for genotyping was extracted (according to conventional methods) from the ear samples of purebred Large White and Landrace ("pure Large White and Landrace" referred to as "pig" in the text of the manual and tables for short). Using the method based on the single-marker association regression model, using the gender of the individual as a fixed effect, the GWAS analysis was performed using the GenABEL software package in ...

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Abstract

The invention belongs to the technical field of animal molecular marker screening, and particularly relates to a SNP molecular marker related to sow leg-foot bone density. The molecular marker is cloned from a gene segment with the registering number of H3GA0030503 and obtained by performing typing and screening on gene through a gene chip. The nucleotide sequence of the molecular marker is shownas SEQ ID NO:1. Allele mutation of G / T at the position of basic group at the 51st position of the sequence, and when nucleotide of the 51st of the SEQ ID NO:1 is G, legs and feet of sows have higher bone density. The invention further discloses a method of screening the molecular marker related to the sow leg-foot bone density and application of a correlation analysis method thereof. A new SNP molecular marker is provided for marker assisted selection of pig leg-foot compactness character especially bone density.

Description

technical field [0001] The invention relates to the technical field of porcine molecular marker preparation, in particular to a molecular marker of a SNP related to the bone density of sow limbs and hooves and its application. The molecular markers described in the present invention can be used to predict the bone density traits of pig limbs and hooves. Background technique [0002] With the development of science and technology, the pig breeding industry has made significant breakthroughs in genetic breeding, nutritional regulation, and disease prevention and control. The lean meat rate, feed-to-meat ratio, and growth rate of commercial pigs have all been greatly improved. However, with intensive farming comes the increasingly prominent problem of limbs and feet. For intensive production, the range of activities of pigs is limited. In addition, the design of pens (slatted floors) has exacerbated the occurrence of pigs' limbs and feet. It has been reported that 20-50% of al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6837C12N15/11
CPCC12Q1/6837C12Q1/6888C12Q2600/124C12Q2600/156
Inventor 刘向东南九红唐振双尹立林叶灯灯马官军赵书红李新云刘小磊
Owner HUAZHONG AGRI UNIV
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