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Method for treating bamboo rat diarrhea by using fecal microbiota transplantation technology

A technology of feces transplantation and bamboo rats, which is applied in the field of bamboo rat plague treatment, can solve the problems of slow effect of traditional Chinese medicine, achieve the effects of less damage to the intestinal system, promote body recovery, and rapid weight gain

Inactive Publication Date: 2018-10-12
NANNING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the actual production process, the effect of traditional Chinese medicine is slow

Method used

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  • Method for treating bamboo rat diarrhea by using fecal microbiota transplantation technology
  • Method for treating bamboo rat diarrhea by using fecal microbiota transplantation technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Select adult female bamboo rats without diarrhea symptoms and grow healthy, and feed them with conditioned feed for 25 consecutive days. 6g, soybean 3g; After 25 days, the feces excreted naturally by bamboo rats were collected immediately after being separated; the collected feces were mixed with 0.3wt.% sodium citrate solution in a mass ratio of 10:1, stirred for 5min, Ferment under aseptic conditions at ℃ for 5 hours, then dilute the fermented fecal bacteria to 5 times with physiological saline, perform ultrasonic treatment, the ultrasonic time is 1min, the frequency is 100Hz, and then filter with an 800-mesh screen to obtain the fermented fecal bacteria liquid; Prepare feed for bamboo rats with diarrhea symptoms, chop 20g carrots, 18g bamboo branches into 2cm, mix with 8g corn kernels, 7g mung beans, 2g peanuts, 0.1g angelica, 0.2g palm, 0.1g jasmine; mix the fermented feces liquid Spray with the feed of the sick bamboo rats at a ratio of 4ml: 200g, stir for 1min, an...

Embodiment 2

[0027] Select adult female bamboo rats that do not suffer from diarrhea symptoms and grow healthy, and feed them with conditioned feed for 28 consecutive days. 8g, soybean 4g; After 28 days, the feces excreted naturally by bamboo rats were collected immediately after being separated; the collected feces were mixed with 0.5wt.% sodium citrate solution in a mass ratio of 10:1, stirred for 5min, Ferment for 4 hours under aseptic conditions at ℃, then dilute the fermented fecal bacteria to 5 times with physiological saline, and perform ultrasonic treatment. Prepare feed for bamboo rats with diarrhea symptoms, chop 23g carrots, 20g bamboo branches into 2cm, mix with 9g corn kernels, 8g mung beans, 3g peanuts, 0.2g angelica, 0.2g palm, 0.1g jasmine; mix the fermented feces liquid Spray with the feed of the sick bamboo rats at a ratio of 5ml: 200g, stir for 2 minutes, and feed the bamboo rats suffering from diarrhea symptoms.

Embodiment 3

[0029] Select adult female bamboo rats without diarrhea symptoms and grow healthy, and feed them with conditioning feed for 30 consecutive days. 9g, soybean 6g; After 30 days, the feces excreted naturally by bamboo rats were collected immediately after being separated; the collected feces were mixed with 0.7wt.% sodium citrate solution in a mass ratio of 10:1, stirred for 5min, Ferment under aseptic conditions at ℃ for 5 hours, then dilute the fermented fecal bacteria to 5 times with physiological saline, perform ultrasonic treatment, the ultrasonic time is 3min, the frequency is 150Hz, and then filter with a 1000-mesh sieve to obtain the fermented fecal bacteria liquid; Prepare feed for bamboo rats with diarrhea symptoms, chop 25g carrots, 22g bamboo branches into 3cm, mix with 10g corn kernels, 9g mung beans, 4g peanuts, 0.3g angelica, 0.2g palm, 0.1g jasmine; mix the fermented feces liquid Spray with the feed of the sick bamboo rats at a ratio of 6ml: 200g, stir for 3 minut...

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Abstract

The present invention relates to the technical field of bamboo rat disease treatment, in particular to a method for treating bamboo rat diarrhea by using a fecal microbiota transplantation technology.According to the method, adult female bamboo rats, as a fecal microbiota source, are continuously fed with corn straw, pennisetum sinese straw, bamboo roots, watermelon peel, sweet potatoes, peanutsand soybeans so as to condition the structure and the number of floras in the intestinal tracts of adult female bamboo rats, and to provide stable and good floras for fecal microbiota used for treating bamboo rat diarrhea, feces in vitro are timely collected, floras in the feces are screened by using sodium citrate and a short-time fermentation method, floras in the feces are extracted and separated to obtain a fecal microbiota liquid, the fecal microbiota liquid is mixed with carrots, bamboo branches, corn particles, mung beans, the peanuts, Chinese angelica, palm and jasmine, and the mixtureis fed to the bamboo rats suffering from diarrhea. The obtained feed is beneficial to the digestion of the diseased bamboo rats, promotes the restoration of intestinal tracts and organisms of the bamboo rats, restores the intestinal function to normal after healing, rapidly increases the weight gain, and reduces the economic loss caused by bamboo rat diarrhea.

Description

technical field [0001] The invention relates to the technical field of treating bamboo rat plague, in particular to a method for treating bamboo rat diarrhea by using fecal bacteria transplantation technology. Background technique [0002] Fecal microbiota transplantation refers to the method of transplanting the functional flora in the feces of healthy individuals into the gastrointestinal tract of patients, rebuilding the intestinal flora with normal functions, and treating diseases inside and outside the gastrointestinal tract. The first use of fecal bacteria transplantation to treat diseases can be traced back to the Eastern Jin Dynasty 1700 years ago. Ge Hong recorded in my first emergency medical book "Elbow Back Emergency Preparedness": to treat food poisoning, diarrhea, fever and dying patients, "Drink one liter of dung juice, that is to live." Current scientific research shows that species differences, growth and development, dietary habits and other factors will a...

Claims

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Application Information

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IPC IPC(8): A23K50/50A23K10/26A23K10/12A23K10/30A23K20/105A61K35/24A61P1/12
CPCA61K35/24A23K10/12A23K10/26A23K10/30A23K20/105A23K50/50A61P1/12Y02P60/87
Inventor 徐高骁
Owner NANNING UNIV
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