Cryopreservation and resuscitation method for ovarian tissue
A technology of ovarian tissue and cryopreservation method, which is applied in the direction of tissue culture, preservation of human or animal body, biochemical equipment and methods, etc., to achieve the effect of good cryopreservation of fertility, ensuring uniformity, and good cell membrane structure
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0096] Example 1-6 Preparation of ovarian tissue cryopreservation protection solution
[0097] Take the preparation of 100ml cryopreservation solution as an example: Measure LeibovitzL-15 medium, DMSO, HSA, SSS and add them to the reagent bottle according to the amount of each component in Table 1. Shake gently to mix, use a 0.22um filter Sterilize by filtration. Store the filter paper in the autoclaved reagent bottle at 4°C for later use, or freeze at -20°C, and move to 4°C refrigerator for later use.
[0098] Table 1 Composition of cryopreservation solution 1
[0099]
Example Embodiment
[0100] Example 7-17 Preparation of ovarian tissue cryopreservation protection solution
[0101] Take the preparation of 100ml cryopreservation solution as an example: Measure LeibovitzL-15 medium, DMSO, HSA, SSS according to the amount of each component in Table 2 and add them to the reagent bottle, shake gently to mix, and then add the corresponding amount Of taurine, nitrogen acetylcysteine, vitamin E, vitamin C. After the solid is completely dissolved, filter and sterilize with a 0.22um filter. The filter paper is autoclaved in a reagent bottle and stored at 4°C for later use, or frozen at -20°C, and moved to a 4°C refrigerator for later use.
[0102] Table 2 Composition of cryopreservation solution 2
[0103]
[0104]
Example Embodiment
[0105] Example 18-20 Preparation of ovarian tissue resuscitation solution
[0106] Take the preparation of 100ml cryopreservation solution as an example: according to the amount of each component in Table 2, measure LeibovitzL-15 medium, DMSO, HSA, SSS into the reagent bottle, shake gently to mix, or add the corresponding The amount of taurine, nitrogen acetylcysteine, vitamin E, vitamin C. After the solid is completely dissolved, filter and sterilize with a 0.22um filter. The filter paper is autoclaved in a reagent bottle and stored at 4°C for later use, or frozen at -20°C, and moved to a 4°C refrigerator for later use.
[0107] Table 3 Composition of resuscitation fluid
[0108]
[0109]
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap