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A wheat plant thousand-grain weight judging marker and its application

A thousand-grain weight and wheat technology, applied in the field of wheat plant thousand-grain weight judgment markers, can solve the problems of different genetic backgrounds, differences in the main effect genes of thousand-grain weight, complex genetic mechanism of thousand-grain weight, etc.

Active Publication Date: 2021-08-27
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the genetic mechanism of 1000-kernel weight is complex, and the main genes controlling 1000-kernel weight are also different in different materials and genetic backgrounds.

Method used

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  • A wheat plant thousand-grain weight judging marker and its application
  • A wheat plant thousand-grain weight judging marker and its application
  • A wheat plant thousand-grain weight judging marker and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Wheat Genomic DNA Extraction

[0031] The experimental materials used for the extraction of wheat genome DNA were parental Jing 411 and Hongmangchun 21, Jing 411 / Hongmangchun 21 populations (F 10) 163 family materials, two high-low mixed pools consisting of 30 families with high thousand-grain weight and 30 families with low thousand-grain weight from Jing 411 / Hongmangchun 21 population, and 369 wheat varieties. Among them, 163 pedigrees from the Jing 411 / Hongmangchun 21 population were used to construct high-density linkage maps and locate main QTLs; A high-low mixed pool composed of 1000-grain weight high and 30 low-1000-grain weight families was used to scan the 660K chip and identify differential SNPs in the main QTL interval; 369 wheat varieties were used to verify the relationship between CAPS markers and thousand-grain weight. 369 wheat varieties were purchased from the market and can be chosen arbitrarily.

[0032] The specific method is: 1. Grind the sing...

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Abstract

The invention belongs to the field of crop genetics and breeding, and provides a marker for judging thousand-grain weight of wheat plants and an application thereof. The marker is a CAPS marker, named JHMfeI, and its sequence is shown in SEQ ID NO.1. The wheat genome was amplified by using marker primers JHMfeI-F: GGAGGAAACAAAGTGGTC, JHMfeI-R: GAGAAGAGGAGGTCGTAG, to obtain amplified products. Among them, wheat plants carrying JHMfeI‑a type (amplified product fragment size of 399bp and 90bp) had higher thousand-grain weight than wheat plants carrying JHMfeI‑b type (amplified product fragment size of 489bp). The CAPS marker JHMfeI developed by the invention can effectively distinguish between high and low thousand-grain weights, is used for high-yield molecular aggregation breeding of wheat, and broadens the genetic basis of wheat breeding materials.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and relates to a marker for judging thousand-grain weight of wheat plants and an application thereof. Background technique [0002] Wheat is the staple food of about 35-40% of the world's population. China is the world's largest wheat producer and consumer, and its annual output accounts for about 17% of the global total. Increasing wheat production is of great strategic significance to my country and global food security. The number of spikes per unit area, the number of grains per spike, and the thousand-grain weight are the three elements that constitute wheat yield. Among them, the thousand-grain weight has relatively high heritability and can be decomposed into basic elements such as grain length, grain width, and grain thickness. Therefore, identifying 1000-grain weight-related loci and developing molecular markers closely linked to them will be helpful for high-yield aggregation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6869C12N15/11
CPCC12Q1/6869C12Q1/6895C12Q2600/13C12Q2600/156C12Q2535/122C12Q2525/191C12Q2531/113
Inventor 张海萍常成谢红咏王升星曹佳佳李黎未文新陈雪健姜昊闵晓宇王建峰卢杰陈璨司红起马传喜
Owner ANHUI AGRICULTURAL UNIVERSITY
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