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Method for analyzing parameter of protein in serum of pneumoconiosis patient among coal miners

A coal worker's pneumoconiosis and parameter analysis technology, applied in the field of protein parameter analysis, can solve the problems of difficult observation and identification of pneumoconiosis, missed treatment period, etc., and achieve the effect of accurate test results, reliable guiding significance, and simple methods

Inactive Publication Date: 2018-10-16
安徽相王医疗健康股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention is aimed at X-ray irradiation in the prior art and needs to be observed and identified by the naked eye of a doctor. It is difficult to observe and distinguish pneumoconiosis in the early stage. Only when the symptoms of pneumoconiosis are obvious can it be distinguished, and at this time the best time has often been missed. To solve the difficult problem in the treatment period, and provide a method for analyzing protein parameters in the serum of patients with coal worker pneumoconiosis

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  • Method for analyzing parameter of protein in serum of pneumoconiosis patient among coal miners

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Experimental program
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Effect test

Embodiment 1

[0030] See attached figure 1 Shown, a kind of coal worker pneumoconiosis patient's serum protein parameter analysis method of the present invention comprises the following steps:

[0031] Step 1. Collection of serum protein cells: First, collect 8ml of venous serum samples from patients diagnosed with coal worker pneumoconiosis; then centrifuge the collected serum samples at 2000rpm and 3°C for 2min to collect the lower layer of cells; then wash with deionized water The collected lower layer cells were centrifuged again at 2000 rpm and 3°C for 2 minutes; finally, the collected lower layer cells were freeze-dried at -60°C for 2 hours to obtain frozen serum protein cells;

[0032]Step 2. Extraction of serum intracellular protein: ①First, place the serum protein cell sample treated in step 1 in a centrifuge tube, add 0.4mL of cell lysate, and mix well; then, add 4 μL with a mass ratio of 2: 1 mixed solution of endodeoxyribonuclease I and endoribonuclease, mix well, and let stand...

Embodiment 2

[0045] See attached figure 1 Shown, a kind of coal worker pneumoconiosis patient's serum protein parameter analysis method of the present invention comprises the following steps:

[0046] Step 1. Collection of serum protein cells: First, collect 9ml of venous serum samples from patients diagnosed with coal worker pneumoconiosis; then centrifuge the collected serum samples at 4000rpm and 4°C for 4min to collect the lower layer of cells; then wash with deionized water The collected lower layer cells were centrifuged again at 4000 rpm and 4°C for 4 minutes; finally, the collected lower layer cells were freeze-dried at -70°C for 5 hours to obtain frozen serum protein cells;

[0047] Step 2. Extraction of serum intracellular protein: ①First, place the serum protein cell sample treated in step 1 in a centrifuge tube, add 0.8mL of cell lysate, and mix well; then, add 12 μL of a mass ratio of 4: 1 mixed solution of endodeoxyribonuclease I and endoribonuclease, mix well, and let stand...

Embodiment 3

[0059] See attached figure 1 Shown, a kind of coal worker pneumoconiosis patient's serum protein parameter analysis method of the present invention comprises the following steps:

[0060] Step 1. Collection of serum protein cells: First, collect 10ml of venous serum samples from patients diagnosed with coal worker pneumoconiosis; then centrifuge the collected serum samples at 6000rpm and 5°C for 2-5min to collect the lower layer of cells; then use deionized Wash the collected lower layer cells with water, and centrifuge again at 6000rpm and 5°C for 5 minutes; finally, freeze-dry the collected lower layer cells at -80°C for 8 hours to obtain frozen serum protein cells;

[0061] Step 2. Extraction of serum intracellular protein: ①First, place the serum protein cell sample treated in step 1 in a centrifuge tube, add 1.2mL of cell lysate, and mix well; then, add 20 μL of a mass ratio of 6: 1 mixed solution of endodeoxyribonuclease I and endoribonuclease, mix well, and stand at 5°...

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Abstract

The invention belongs to the field of analysis of the protein parameter and discloses a method for analyzing the parameter of the protein in the serum of a pneumoconiosis patient among coal miners. The method comprises the following steps: collecting a serum protein cell, extracting the protein in the serum cell, and implementing liquid phase mass spectrometry analysis on the parameter of the protein; the method firstly collects the serum protein cell, then extracts the protein in the serum cell, and finally tests the parameter of the protein in the serum cell through the liquid chromatography, the parameter of the protein in the serum cell is accurately quantitatively qualitatively tested, the testing result is accurate and has quite reliable guiding significance when the being used in the detection, and the pneumoconiosis of the coal miner is effectively prevented and diagnosed.

Description

technical field [0001] The invention relates to the technical field of protein parameter analysis, in particular to a method for analyzing protein parameters in serum of coal worker pneumoconiosis patients. Background technique [0002] The standard name of pneumoconiosis is pneumoconiosis, which is a systemic disease mainly caused by diffuse fibrosis of lung tissue caused by long-term inhalation of productive dust and dust in occupational activities and retention in the lungs. However, due to the special reasons of their work, coal miners are exposed to coal mine dust for a long time, which is very easy to cause pneumoconiosis. However, pneumoconiosis has no specific clinical manifestations, and its clinical manifestations are mostly related to comorbidities, such as ①cough: the cough of early pneumoconiosis patients is not obvious, but with the progress of the disease, patients often have chronic bronchitis, and late patients often have pulmonary Infection can make cough ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N21/78
CPCG01N21/78G01N30/02
Inventor 许希海黄亚北柴红修韩颖孙斌孙秀霞高伟
Owner 安徽相王医疗健康股份有限公司