A method for detecting copper ions using high-throughput photoelectric colorimetry
A technology of copper ion and colorimetry, which is applied in the field of chemical colorimetry detection, can solve the problems of cumbersome operation steps and high cost, and achieve the effect of low quality requirements, short time consumption and simple operation
Active Publication Date: 2021-07-30
NANJING UNIV
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Problems solved by technology
However, the commonly used colorimetric method is the gold gel colorimetric method, which has high cost and relatively cumbersome operation steps, which need to be further simplified.
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[0035] step 1)
[0036] Preparation of hydrochloric acid doped intrinsic polyaniline (EB-PANI).
[0037] Take 250 μL of EB-PANI dimethyl sulfoxide solution, mix with 250 μL of 1.0M hydrochloric acid, and place in a 35°C incubator to shake overnight. After shaking, centrifuge at 7000 rpm for 10 minutes and wash with 600 μL ultrapure water. After washing, centrifuge at 7000 rpm for 10 min, remove the supernatant, and finally dilute to volume with 300 μL ultrapure water. After constant volume is the prepared ES-PANI solution, which can be stored at room temperature for more than one week.
[0038] step (2)
[0039] Take 5μL 1mg·mL -1 TiO 2 The aqueous suspension of the solution and 5 μL of the ES-PANI solution prepared by step (1) were thoroughly mixed and added to a 384-well plate, and then 10 μL of Cu with known concentration was added 2+ solution, mix well.
[0040] step (3)
[0041] The above-mentioned 384-well plate was placed in a 35°C incubator, and an ultraviolet ...
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Abstract
The invention relates to a method for detecting copper ions by high-throughput photoelectric colorimetry, which uses polyaniline as a chromogenic substrate, comprising: step 1: preparing polyaniline in an intermediate oxidation state doped with hydrochloric acid; step 2: Mix titanium dioxide with polyaniline in the intermediate oxidation state, and add an appropriate amount to a 384-well plate to form polyaniline in the intermediate oxidation state and TiO 2 The mixed solution; step three: adding the solution to be detected containing divalent copper ions of unknown concentration to the mixed solution obtained in step two, and using a pipette gun to mix the mixed solution with the solution to be detected; step Four: Place the 384-well plate carrying the solution obtained in step three under ultraviolet light; step five: visually distinguish the color reaction caused by divalent copper ions, titanium dioxide and polyaniline under ultraviolet light irradiation The color change gives an initial readout of the concentration range. Thus, rapid, low-cost and simple detection of the concentration of trace copper ions is realized.
Description
technical field [0001] The invention generally relates to the field of chemical colorimetric detection, and in particular to a method for detecting copper ions by using a high-throughput photoelectric colorimetric method. Background technique [0002] Copper is an essential nutrient element for the human body. Copper deficiency will affect intellectual development and bone development, but excessive intake will cause heavy metal ion poisoning, leading to serious diseases. Therefore, the control of copper intake is particularly important. The sources of copper ions ingested by the human body are mainly water, food and the environment, so it is very important to measure the trace amount of copper ions from food and water. The United States Environmental Protection Agency stipulates that the content of copper ions in drinking water should not exceed 20 μM. [0003] At present, the detection methods of trace copper mainly include fluorescence method, spectrophotometry, electro...
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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 雷建平杨芊荟郝青鞠熀先
Owner NANJING UNIV