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System, method and computer readable medium for background compensation of digital images

A technology of digital image and background compensation, applied in the field of background compensation and DNA/RNA sequencing, which can solve the problems of reducing information accuracy and intensity attenuation

Active Publication Date: 2022-06-24
QIAGEN GMBH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Furthermore, these intensities are additionally attenuated by the halo effect caused by the optics and uneven illumination
In conventional systems without background compensation, these effects lead to a reduction in the precision of the information obtained from the images, i.e. the precision of base calling in DNA / RNA sequencing

Method used

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  • System, method and computer readable medium for background compensation of digital images
  • System, method and computer readable medium for background compensation of digital images

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Embodiment approach

[0048] figure 1 The system of the present invention is shown. According to an exemplary embodiment of the present invention, the system includes an intensity determination unit 100 and a calculation unit 200 . The unit is configured to perform one or more steps of the present invention. Although the invention has been described using two separate units 100, 200, it is clear that separate units may also be part of a single "main" unit.

[0049] In a preferred embodiment of the present invention, the intensity determination unit 100 is configured to determine a first intensity value of each object and a second intensity value of at least one second object within a plurality of predetermined regions from the digital image.

[0050] The calculation unit 200 is configured to calculate a median intensity value for each of the plurality of predetermined regions, calculate an interpolation of the median intensity value to the original digital image size, and by subtracting the media...

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Abstract

The present invention relates to systems, methods and computer-readable media for background compensation of digital images comprising at least one first object and at least one second object, the at least one first object having received genetic information containing At least one molecule, the at least one second subject did not receive a molecule comprising genetic information, the at least one molecule is configured to receive a fluorescent compound, the digital image is determined by the optical imaging system during emission of electromagnetic radiation by the fluorescent compound.

Description

technical field [0001] The present invention relates to systems and methods for background compensation of digital images comprising at least one first object and at least one second object. More particularly, the present invention relates to background compensation of digital images determined by optical imaging systems during emission of electromagnetic radiation by fluorescent compounds, preferably for DNA / RNA sequencing, where autofluorescence effects play a role. Background technique [0002] Biotechnology, medicine and related technical fields are based on molecular analysis. Electronics can analyze molecules with high precision and specificity. Especially in the past few years, automated electronics have been developed to analyze large numbers of samples by conventional methods. For example, modern DNA sequencing equipment is used for routine analysis of large numbers of DNA probes. Protein samples can be analyzed by high-throughput screening and related methods. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06T5/00
CPCG06T2207/10064G06T2207/20021G06T2207/30072G06T5/94G06T5/20G06T5/50
Inventor 托斯滕·泽尔法斯比约恩·拉比茨克迈科·洛赫尔
Owner QIAGEN GMBH