Application of microrna98 in the preparation of drugs for the treatment of ischemic stroke
A microrna98, ischemic stroke technology, applied in the field of genetic engineering and drug development
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Embodiment 1
[0018] Example 1: Preparation of an animal model of focal cerebral ischemia-reperfusion.
[0019] According to the Longa method, the right middle cerebral artery occlusion (middle cerebral artery) in the monofilament lumen of rats was prepared by using a nylon suture (model: 2634-A4, Beijing Xinong Technology Co., Ltd., China) with the head end coated with polylysine. Arteriocclusion, MCAO) model. SD rats were fasted for 8-10 hours before the operation, anesthetized by intraperitoneal injection of 10% chloral hydrate (0.4ml / 100g, prepared in sterile saline), fixed in the supine position, and made a median neck incision after povidone-iodine disinfection, and separated the right side The common carotid artery, internal carotid artery and external carotid artery were inserted into the clot thread from the incision of the external carotid artery until resistance (approximately 20 mm) appeared, indicating that the clot thread had reached the middle cerebral artery, and the clot th...
Embodiment 2
[0020] Embodiment 2: Experimental grouping and administration.
[0021] SD rats were randomly divided into groups after weighing: sham operation group (Sham-Vehicle), cerebral ischemia model group, and microRNA98 treatment group.
[0022] Administration method: According to the weight of the rat, intraperitoneal injection of 10% chloral hydrate (4.0ml / kg) was anesthetized, and the prone position was fixed to keep the skull horizontally in the middle. Shearing, disinfecting with iodine, exposing the top of the skull, dipping an appropriate amount of H 2 o 2 Wipe the top of the skull to expose the bregma. According to the rat brain atlas, the coordinates of the lateral ventricle are (AP: 0.8mm, ML: 1.5mm, DV: 4.0mm), drill the dura mater according to the coordinate position, insert the needle vertically to the predetermined coordinate position, and inject the drug slowly (approx. 1ul / min), the needle was retained for 10-15 minutes after the injection was complete. Slowly rai...
Embodiment 3
[0023] Example 3: Scoring of neuromotor function.
[0024] After 24 hours of operation, the neurological deficits of the rats were graded and scored according to the Bederson method, and the rats whose neurological function score was 0 at 24 hours were removed, and the mean value of the scores was calculated. The standards are as follows:
[0025] 0 points: normal, no neurological symptoms observed;
[0026] 1 point: When the tail is suspended in the air, the contralateral forelimb of the animal cannot be fully extended, showing wrist and elbow flexion, shoulder internal rotation, elbow abduction, and close to the chest wall;
[0027] 2 points: Place the animal on a smooth surface, and when the animal walks freely, it circles or circles to the opposite side of the operation;
[0028] 3 points: when the animal walks freely, it falls to the side opposite to the operation;
[0029] 4 points: The animal is placed on a smooth surface, without spontaneous walking, accompanied by ...
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