A method of improving salt resistance of soybean seedling stage
A technology of salt resistance and soybean seedlings, which is applied in the field of crop science, can solve the problem of salt resistance of soybean seedlings that have not yet been soaked in chemical reagents for pretreatment
Active Publication Date: 2021-07-06
FUJIAN AGRI & FORESTRY UNIV
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At present, there is no method for soaking soybean seeds in chemical reagents to improve their salt resistance at the seedling stage
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[0023] A method for improving the salt resistance of soybean seedlings, specifically comprising the following steps:
[0024] (1) Preparation of pretreatment solution: the formula of the pretreatment solution is: 5mM KNO 3 , 5mM Ca(NO 3 ) 2 ·5H 2 O, 5wt% PEG, 1.5mM Na 2 SiO 3 9H 2 O;
[0025] (2) Select plump soybean seeds and clean them for later use;
[0026] (3) Soak the cleaned soybean seeds in a pretreatment solution 5.5 times the volume of the seeds, and soak at room temperature for 17 hours;
[0027] (4) Remove the soaking solution and use it directly for the detection of soybean seed germination or the detection of salt resistance of soybean seedlings.
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The invention discloses a method for improving the salt resistance of soybean seedlings. The cleaned soybean seeds are soaked in a pretreatment solution, and soaked at room temperature for 16-18 hours; Salt resistance detection of seedlings; the formula of the pretreatment solution is: 5mM KNO 3 , 5mM Ca(NO 3 ) 2 ·5H 2 O, 5 wt% PEG, 1.5 mM Na 2 SiO 3 ·9H 2 O. Compared with the traditional breeding of soybean salt-resistant varieties, transgenic soybean salt-resistant, spraying exogenous reagents in the growth period and some schemes of using Agrobacterium co-cultivation to improve soybean salt-resistant, the method of the invention has a short time and is simple and easy. Yes, there are no concerns about the safety of genetically modified foods, and it has significantly improved tolerance to salt.
Description
technical field [0001] The invention belongs to the technical field of crop science, and in particular relates to a method for improving the salt resistance of soybean seedlings. Background technique [0002] Cultivated soybean (Glycine max) is an important grain and oil crop. It also contains secondary metabolites such as soybean isoflavones that are beneficial to human health, and have high practical value. Since leguminous plants have nitrogen-fixing rhizobia, they can fertilize the field to a certain extent, which is beneficial to the soil restoration of cultivated land, and are often selected for soil improvement. Cultivated soybeans are moderately salt-resistant crops. Salt stress has many adverse effects on soybean growth and various agronomic traits, hindering the biological nitrogen fixation process of soybean rhizobia, and ultimately reducing yield and quality. Due to climate change, irrigation of various fertilizers and pesticides, seawater intrusion, etc., 20% o...
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IPC IPC(8): A01C1/00A01N59/06A01N59/00A01N31/02A01P21/00
CPCA01C1/00A01N31/02A01N59/00A01N59/06
Inventor 贾琪孙松尚明明郑程文梁康迳
Owner FUJIAN AGRI & FORESTRY UNIV
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