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Preparation method of matrix standard substance of aflatoxin b1 in peanut

A kind of technology of aflatoxin and standard substance, which is applied in the field of preparation of aflatoxin B1 matrix standard substance in peanuts, can solve the problems of many bacteria, difficult control of sample uniformity and stability, uneven toxin production, etc. Bacterial influence, operability improvement effect

Active Publication Date: 2021-03-23
NAT INST OF METROLOGY CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural toxin-contaminated peanut raw materials often need to be obtained through large-scale sample screening. Due to uncontrollable toxin production conditions and excessive bacteria, it is more likely to lead to uneven toxin production, and it is difficult to control the uniformity and stability of samples. There are also many limitations in terms of sample volume, sample concentration control, etc.

Method used

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  • Preparation method of matrix standard substance of aflatoxin b1 in peanut
  • Preparation method of matrix standard substance of aflatoxin b1 in peanut
  • Preparation method of matrix standard substance of aflatoxin b1 in peanut

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Embodiment 1

[0032] Such as figure 1 Shown, a kind of aflatoxin B1 matrix standard substance preparation method in peanut, comprises the following steps:

[0033] (1) Selection of toxin-producing strains

[0034] A strain of Aspergillus flavus (Aspergillus flavus) with the strain number CGMCC No3.4408 was purchased from the General Microbiology Center (CGMCC) of the China Committee for Culture Collection of Microorganisms. It has been confirmed by peanut toxicity experiments that the strain can efficiently produce aflatoxin B1.

[0035] (2) Selection of blank and toxin-producing peanut varieties

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Abstract

The invention discloses a preparation method of aflatoxin B1 matrix reference material in peanut. The preparation method comprises: (1) selection of an aflatoxin B1 production strain; (2) selection ofa blank variety and an aflatoxin B1 production peanut variety; (3) peanut sample treatment and aflatoxin B1 production monitoring; (4) matrix mixing and preparation; and (5) sample characterization and uniformity inspection. With the process of the invention, the aflatoxin generation concentration in the peanut can be controlled so as to improve the stability and the uniformity of the sample.

Description

technical field [0001] The invention relates to the technical field of chemical standard substance preparation, in particular to a preparation method of aflatoxin B1 matrix standard substance in peanuts. Background technique [0002] Aflatoxin contamination is one of the major problems in food safety and seriously threatens human health. The development of aflatoxin matrix reference materials is the basis for ensuring the accuracy and reliability of relevant food safety testing data. Among the most common aflatoxins in peanut seeds, aflatoxin B1 is the most toxic and the most toxic. Because peanuts have the characteristics of high fat and high protein, and the distribution of aflatoxin-producing bacteria in peanuts is uneven, and the toxin production is uneven, the uniformity and stability control of samples is the key to the development of standard materials. Natural toxin-contaminated peanut raw materials often need to be obtained through large-scale sample screening. Du...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/30G01N1/38G01N1/44C12P17/18C12R1/67
CPCC12P17/181G01N1/28G01N1/286G01N1/30G01N1/38G01N1/44G01N2001/2866G01N2001/2873
Inventor 卢晓华张磊王阳
Owner NAT INST OF METROLOGY CHINA