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Coconut embryo induction medium and method for obtaining in vitro regenerated plants based on thin-layer culture of coconut zygotic embryo cells

A technology of induction medium and thin-layer culture, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of low reproduction coefficient, separation of traits of hybrid offspring, long growth cycle, etc., to achieve short reproduction cycle, Controllable, easy-to-cultivate effects

Active Publication Date: 2020-11-13
HAINAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, coconuts are mostly cross-pollinated plants with long growth cycle, low reproduction coefficient, serious separation of traits in hybrid progeny, and large inter-individual differences. It is difficult to obtain offspring groups with the same traits. Therefore, conventional vegetative propagation methods such as cuttings, grafting, and high-altitude layering cannot be used for asexual reproduction of coconuts. Therefore, it is necessary to establish a complete, efficient and practical coconut tissue culture and rapid propagation technology system to realize the cultivation of excellent clonal strains of coconuts. Provide technical support for the promotion of large-scale breeding of high-quality coconut tissue culture seedlings
[0004] At present, there is no successful plant regeneration case for coconut tissue culture technology, and we can only explore and study the plant regeneration technology of other species. However, the genetic background and living environment of different species are very different. R&D helps little

Method used

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  • Coconut embryo induction medium and method for obtaining in vitro regenerated plants based on thin-layer culture of coconut zygotic embryo cells
  • Coconut embryo induction medium and method for obtaining in vitro regenerated plants based on thin-layer culture of coconut zygotic embryo cells

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Experimental program
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Effect test

Embodiment 1

[0094] A kind of coconut embryo induction culture medium, take the improved Y3 culture medium (Y3*) as basic culture medium, and described improved Y3 culture medium is to adjust following component content on the basis of international general Y3 culture medium: 0.025 mg / L CuSO 4 ·5H 2 O, 56mg / L Na 2 EDTA and 12mg / L FeSO 4 ·7H 2 O; Simultaneously, 1mg / L dicamba, 1mg / L NAA, mass concentration of 3% sucrose, mass concentration of 0.35% agar and mass concentration of 0.1% gac are also included in the basic medium; the pH of the coconut embryo induction medium The value is 5.5. Prepare and sterilize to obtain coconut embryo induction medium.

Embodiment 2

[0096] A kind of coconut embryo induction culture medium, with improved Y3 culture medium (Y3*) as basic culture medium, described improved Y3 culture medium is to adjust following component content on the basis of international general Y3 culture medium: 0.25 mg / L CuSO 4 ·5H 2 O, 25mg / L Na 2 EDTA and 45mg / L FeSO 4 ·7H 2 O; at the same time, the basic medium contains 0.3mg / L 2,4D, 0.8mg / L dicamba, 0.3mg / L NAA, the mass concentration is 6% sucrose, the mass concentration is 0.4% agar and the mass concentration is 0.18%~0.23 % activated carbon, pH 5.4. Prepare and sterilize to obtain coconut embryo induction medium.

Embodiment 3

[0098] A kind of coconut embryo induction culture medium, with improved Y3 culture medium (Y3*) as base culture medium, described improved Y3 culture medium is to adjust following component content on the basis of international general Y3 culture medium: 0.1 mg / L CuSO 4 ·5H 2 O, 40mg / L Na 2 EDTA and 30mg / L FeSO 4 ·7H 2 O; At the same time, the basic medium also contains 0.5mg / L 2,4D, 0.5mg / L dicamba, 0.5mg / L NAA, the mass concentration is 4.2% sucrose, the mass concentration is 0.4% agar and the mass concentration is 0.18% activated carbon ; The pH value of the coconut embryo induction medium is 5.6. Prepare and sterilize to obtain coconut embryo induction medium.

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Abstract

The invention provides a coconut embryo inducing medium and a method for obtaining an in-vitro regeneration plant based on coconut zygotic embryo thin cell layer culture, and belongs to the technicalfield of coconut tissue culture. The coconut embryo inducing medium adopts an improved Y3 medium as a basal medium; the basal medium is also prepared from 2,4-D, dicamba, NAA (Naphthalene Acetic Acid), cane sugar, agar and activated carbon; a pH value of the coconut embryo inducing medium is 5.5 to 5.8. By adopting a coconut zygotic embryo as an explant, and through the processes such as explant sterilization, thin cell layer culture, embryo induction, enrichment culture, rooting culture, and acclimatization and transplant, a coconut tissue culture and rapid propagation system is built, high-quality coconut tissue culture seedlings are sustainably provided for the coconut industry, and the system has a great significance on promoting upgrade and update of the coconut industry.

Description

technical field [0001] The invention belongs to the technical field of coconut tissue culture, and in particular relates to a coconut embryo induction medium and a method for obtaining in vitro regenerated plants based on thin layer culture of coconut zygote embryo cells. Background technique [0002] Cocos (Cocos nucifera L.) is a perennial tree of the genus Cocos in the palm family. It is a typical tropical woody oil crop and food crop, and an important economic crop in the tropical regions of the world. At present, the output of coconut in my country can only meet about 10% of the total demand, and the contradiction between supply and demand is prominent. Therefore, it is urgent to accelerate the development of coconut planting industry. [0003] However, coconuts are mostly cross-pollinated plants, with long growth cycle, low reproduction coefficient, severe separation of traits in hybrid progeny, and large inter-individual differences. It is difficult to obtain offsprin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 尤丽莉
Owner HAINAN UNIV