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Method for extracting blood-pressure-lowering active peptide from casein

A blood pressure-lowering peptide and casein technology, which is applied in the field of food-derived active peptide development, can solve the problems of cumbersome and time-consuming separation methods of blood pressure-lowering active peptides, and achieve good social promotion and application, simple process, and rich raw materials.

Inactive Publication Date: 2018-11-13
马学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the prevalence of hypertension and the importance of safe antihypertensive treatment, milk casein peptides, as the antihypertensive drug components derived from natural food, are more likely to be accepted by hypertensive people, but the existing isolation methods for antihypertensive active peptides are cumbersome and time consuming

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for extracting antihypertensive peptides from casein, including the following steps:

[0028] Step 1: First take a certain amount of milk or casein to dissolve in water, then adjust the concentration to 7%, and stir for 5 minutes after completion, until the milk or casein is completely denatured and dissolved to obtain a casein solution;

[0029] Step 2: Cool the casein solution to room temperature, first adjust the pH of the casein solution to 5.5, and then add pepsin to the casein solution to carry out the hydrolysis reaction, maintain the reaction temperature at 20°C, hydrolyze for 1 hour, and obtain the enzyme after the enzyme is inactivated Solution solution

[0030] Step 3: Then add trypsin to the enzymolysis solution, maintain the reaction temperature at 30℃, and hydrolyze for 3h;

[0031] Step 4: Then the temperature is raised to 95°C and maintained for 10 minutes to inactivate the trypsin to obtain a secondary enzymatic hydrolysis solution;

[0032] Step 5: Cen...

Embodiment 2

[0040] A method for extracting antihypertensive peptides from casein, including the following steps:

[0041] Step 1: First take a certain amount of milk or casein and dissolve it in water, then adjust the concentration to 8%, and stir for 6 minutes after completion, until the milk or casein is completely denatured and dissolved to obtain a casein solution;

[0042] Step 2: Cool the casein solution to room temperature, first adjust the pH of the casein solution to 6.5, then add pepsin to the casein solution to carry out the hydrolysis reaction, maintain the reaction temperature at 30°C, hydrolyze for 2 hours, and obtain the enzyme after inactivation Solution solution

[0043] Step 3: Then add trypsin to the enzymolysis solution, maintain the reaction temperature at 30~60℃, and hydrolyze for 3.5h;

[0044] Step 4: Then the temperature is raised to 100°C and maintained for 15 minutes to inactivate the trypsin to obtain a secondary enzymatic hydrolysis solution;

[0045] Step 5: Centrifug...

Embodiment 3

[0053] A method for extracting antihypertensive peptides from casein, including the following steps:

[0054] Step 1: First take a certain amount of milk or casein and dissolve it in water, then adjust the concentration to 9%, and stir for 7 minutes after completion, until the milk or casein is completely denatured and dissolved to obtain a casein solution;

[0055] Step 2: Cool the casein solution to room temperature, first adjust the pH of the casein solution to 7.5, then add pepsin to the casein solution to carry out the hydrolysis reaction, maintain the reaction temperature at 40°C, hydrolyze for 3 hours, and obtain the enzyme after inactivation Solution solution

[0056] Step 3: Then add trypsin to the enzymolysis solution, maintain the reaction temperature at 50℃, and hydrolyze for 4h;

[0057] Step 4: Then the temperature is raised to 105°C and maintained for 20 minutes to inactivate trypsin to obtain a secondary enzymatic hydrolysis solution;

[0058] Step 5: Centrifuge the sec...

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Abstract

The invention discloses a method for extracting a blood-pressure-lowering active peptide from casein and relates to the technical field relates to food-borne active peptide development. The method comprises ten steps, a two-step enzymolysis method is adopted, and antihypertensive peptide having higher activity can be simple, conveniently and quickly. Compared with synthesized blood-pressure-lowering products, the blood-pressure-lowering active peptide is high in eating safety and free of toxic or side effect, has the blood-pressure-lowering effect on a hypertensive, but has no pressure-lowering effect on normal persons. The method can be widely applied to drugs, health-care foods and food additives for treating cardiovascular diseases. The biological active peptide is prepared by adoptingcow's milk or casein as raw materials, the raw materials are very rich, the price is low, large-scale industrial production can be achieved, and the method has the important significance in cow's milkproduct diversification and development and utilization of the casein biological active peptide in functional foods, health-care foods or drugs.

Description

Technical field [0001] The present invention relates to the technical field related to the development of food-derived active peptides, in particular to a method for extracting blood pressure-lowering active peptides from casein. Background technique [0002] A large number of studies have found that protein is not completely absorbed by the body in the form of amino acids in the human body, but is absorbed by the body in the form of small molecular peptides. These small molecule peptides composed of several amino acids not only have nutritional functions and physiological activities, but also have certain functional properties, such as anti-oxidation, antibacterial, and immunity enhancement. [0003] Antihypertensive peptide is such a small molecule peptide with the function of lowering blood pressure. Its molecular weight is mainly about 1500u. It is an inhibitor of Angiotension-converting Enzyme (ACE), so it is also called ACE inhibitory peptide ( Angiotension-converting Enzyme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/47C07K1/36C07K1/34C07K1/16A61P9/12
CPCC12P21/06A61P9/12C07K14/4732
Inventor 马学
Owner 马学