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A kind of sulfur oxidizing bacteria cultivation and activity enhancement method and application

A technology of sulfur oxidizing bacteria and culture method, which is applied in the field of sulfur oxidizing bacteria culture and activity enhancement, halophilic alkaliphile thiaalkalivibrio culture and activity enhancement field, which can solve undisclosed problems and achieve the improvement of desulfurization efficiency and sulfur conversion ability The effect of promoting and accelerating the growth rate

Active Publication Date: 2021-11-02
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, how to improve the growth rate and desulfurization rate of halophilic and alkaline sulfur-oxidizing bacteria is not disclosed in this patent

Method used

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  • A kind of sulfur oxidizing bacteria cultivation and activity enhancement method and application
  • A kind of sulfur oxidizing bacteria cultivation and activity enhancement method and application
  • A kind of sulfur oxidizing bacteria cultivation and activity enhancement method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 bacterial cell culture

[0038] Thioalkalivibrio versatus D301 is a halophilic, alkaliphilic, sulfur-oxidizing bacterium isolated from a saline lake in Inner Mongolia, China.

[0039] Add 1 mL of the strain stored at -80°C to 100 mL of mineral liquid medium, culture at 30°C and 180 rpm for 24 hours, and the sulfur source is sodium thiosulfate to obtain seeds;

[0040] Take 5mL of the cultured seed bacteria liquid, centrifuge at 8000rpm for 10min, inoculate in the liquid medium, and cultivate at 30°C and 180rpm for 24h, wherein the Na in the liquid medium + The concentration is 1.3M, SO 4 2- The concentration is 30g / L.

Embodiment 2

[0041] Embodiment 2 bacterial cell culture

[0042] Add 1 mL of the strain stored at -80°C to 100 mL of mineral liquid medium, culture at 30°C and 180 rpm for 24 hours, and the sulfur source is sodium thiosulfate to obtain seeds;

[0043] Take 5mL of the cultured seed bacteria liquid, centrifuge at 8000rpm for 10min, inoculate in the liquid medium, and cultivate at 30°C and 180rpm for 24h, wherein the Na in the liquid medium + The concentration is 1.6M, SO 4 2- The concentration is 30g / L.

Embodiment 3

[0044] Embodiment 3 bacterial cell culture

[0045] Add 1 mL of the strain stored at -80°C to 100 mL of mineral liquid medium, culture at 30°C and 180 rpm for 24 hours, and the sulfur source is sodium thiosulfate to obtain seeds;

[0046] Take 5mL of the cultured seed bacteria liquid, centrifuge at 8000rpm for 10min, inoculate in the liquid medium, and cultivate at 30°C and 180rpm for 24h, wherein the Na in the liquid medium + The concentration is 1.6M, SO 4 2- The concentration is 50g / L.

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Abstract

The present invention provides a sulfur oxidizing bacteria culture and activity enhancement method and its application. The sulfur oxidizing bacteria culture method comprises inoculating sulfur oxidizing bacteria in a liquid medium for cultivation; wherein, in the liquid medium, Na + The concentration is 1.0M‑2.5M, SO 4 2‑ The concentration is 10g / L‑100g / L. The present invention adds an appropriate amount of SO to the sulfur-oxidizing bacteria culture medium 4 2‑ , which can promote the growth of sulfur oxidizing bacteria and the sulfur conversion ability of sulfur oxidizing bacteria, with the SO 4 2‑ With the increase of concentration, the growth rate of sulfur oxidizing bacteria is accelerated, and the desulfurization efficiency is improved; the added SO 4 2‑ It triggers the adjustment of sulfur-oxidizing bacteria at the level of gene transcription and protein translation, which is more conducive to the growth of sulfur-oxidizing bacteria and the transformation of sulfur substances in a high-salt environment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method and application of sulfur-oxidizing bacteria cultivation and activity enhancement, in particular to a method and application of halophilic and alkaliphilic thioalkali vibrio cultivation and activity enhancement. Background technique [0002] Hydrogen sulfide is a highly toxic and highly corrosive gas with a foul smell, widely present in natural gas, biogas, and waste gas and wastewater from refineries. Hydrogen sulfide has a serious corrosive effect on drilling equipment and transportation pipelines, and has a lethal effect on the human body in the case of extremely low concentration leakage, and its oxidation product sulfur dioxide has a polluting effect on the atmosphere. In recent years, with the implementation of my country's winter heating coal-to-gas and coal-to-electricity policies, natural gas has become an increasingly important clean energy source; in addition, the s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P3/00C12R1/63
CPCC12N1/20C12P3/00
Inventor 邢建民郝学密杨茂华穆廷桢江启沛
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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