Method for extracting zingiberensis newsaponin

A technology of echinoside and compound recipe, which is applied in the field of extracting echinocoside, can solve the problems of low extraction rate of the preparation method, and achieve the effects of high extraction rate, fast extraction speed and high purity

Inactive Publication Date: 2018-11-20
江苏黄河药业股份有限公司
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention also relates to the use of turmeric psilocybin in the preparation of medicines for the treatment or prevention of cancer, wherein the cancer is sele

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Example 1

[0026] A method for extracting scutellarin, comprising the following steps:

[0027] (1) Wash, dry, pulverize the rhizomes of the medicinal plant Dioscorea officinalis, weigh the rhizome powder, add the active agent aqueous solution, supersonicate under ultrasonic conditions, and supersonicate for 40min under the condition of ultrasonic frequency of 25.8KHZ, centrifuge at 8000-12000r / min Centrifuge for 10 min, take the supernatant for suction filtration; in this step, add 10 g of rhizome powder, add 100 ml of aqueous surfactant solution to obtain an aqueous solution of sodium fatty acid methyl ester sulfonate, and the concentration of the aqueous surfactant solution is 8.6 × 10 -3 mol / l. The mesh number of rhizome powder is 80 meshes.

[0028] (2) After suction filtration, add 70-100% (volume ratio) ethanol for leaching for 10-24 hours, and separate the solid and liquid;

[0029] (3) Add 4 times the weight of 70-100% ethanol to the solid phase separated ...

Example Embodiment

[0035] Example 2

[0036] A method for extracting scutellarin, comprising the following steps:

[0037] (1) Wash, dry, pulverize the rhizomes of the medicinal plant Dioscorea officinalis, weigh the rhizome powder, add the active agent aqueous solution, supersonicate under ultrasonic conditions, supersonicate for 40 minutes under the condition of ultrasonic frequency of 20KHZ, and centrifuge at 8000-12000r / min Centrifuge for 10 min, and take the supernatant for suction filtration; in this step, add 10 g of rhizome powder, add 80 ml of aqueous surfactant solution to obtain aqueous solution of sodium dodecyl sulfonate, and the concentration of aqueous surfactant solution is 5.0×10 -3 mol / l. The mesh number of rhizome powder is 80 meshes.

[0038] (2) After suction filtration, add 70-100% (volume ratio) ethanol for leaching for 10-24 hours, and separate the solid and liquid;

[0039] (3) Add 3 times the weight of 70-100% ethanol to the solid phase separated by filtration, reflu...

Example Embodiment

[0045] Example 3

[0046] A method for extracting scutellarin, comprising the following steps:

[0047] (1) Wash, dry, pulverize the rhizomes of the medicinal plant Dioscorea officinalis, weigh the rhizome powder, add the active agent aqueous solution, supersonicate under ultrasonic conditions, supersonicate for 40min under the condition of ultrasonic frequency of 30KHZ, and centrifuge at 8000-12000r / min Centrifuge for 10 min, and take the supernatant for suction filtration; in this step, add 10 g of rhizome powder to 80 ml of aqueous surfactant solution to obtain an aqueous solution of sodium dodecylbenzenesulfonate, and the concentration of the aqueous surfactant solution is 10.0×10 -3 mol / l. The mesh number of rhizome powder is 80 meshes.

[0048] (2) After suction filtration, add 70-100% (volume ratio) ethanol for leaching for 10-24 hours, and separate the solid and liquid;

[0049] (3) Add 3 times the weight of 70-100% ethanol to the solid phase separated by filtration...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Meshaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for extracting zingiberensis newsaponin. A method of combining a aqueous surfactant solution and an organic solvent is adopted, extraction is performing with the assist of ultrasonic, and the method comprises the following steps: washing, drying and pulverizing rhizomes and stems of a medicinal plant, dioscorea nipponica, weighing powder of the rhizomes and stems,adding into an active agent aqueous solution, performing ultrasonic treatment under an ultrasonic condition, centrifuging, taking supernatant, and performing suction filtration; adding ethyl alcohol after the suction filtration for leaching and extraction, and performing solid-liquid separation; adding a filtered and separated solid phase into ethyl alcohol for reflux; combining the socked liquidphase with the liquid phase extracted by the hot reflux, distilling under reduced pressure, recycling ethyl alcohol, and obtaining brown extract; adding water into the brown extract, and performing ultrasonic dissolution; centrifuging the obtained mixture to separate the aqueous phase and the solid phase; freeze-drying the obtained solid phase to obtain total steroidal saponin components of rhizoma zingiberis; performing separation and purification with normal phase silica gel column chromatography, gel chromatography and recrystallization to obtain the zingiberensis newsaponin. A novel methodfor extracting zingiberensis newsaponin is adopted, the extraction speed is high, the extraction rate of zingiberensis newsaponin is high, and the purity is high.

Description

technical field [0001] The invention belongs to the technical field of extracting traditional Chinese medicines, and in particular relates to a method for extracting glucoside. Background technique [0002] Malignant tumors, as one of the largest public health problems in the world, have greatly endangered human health and will become the number one killer of human beings in the new century. From a global perspective, developing countries are facing a greater burden of disease. As a large developing country, my country is facing an increasingly severe situation with malignant tumors. [0003] Dioscin (C 51 h 82 o 2 ) class chemical constituents are the main effective chemical constituents in Dioscorea scutellaria. Modern pharmacological studies have shown that such chemical components have biological activities such as anti-inflammatory, anti-tumor, hypolipidemic, and immune regulation. Di'ao Xinxuekang Capsules and Xinnaoshutong Capsules commonly used in clinical pract...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07J71/00A61P35/00
CPCA61P35/00C07J71/0005
Inventor 朱正标闫海粟殷媛媛范小雪陶元明
Owner 江苏黄河药业股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap