Compound microbial agent and microbial fertilizer
A technology of compound microbial inoculants and microbial fertilizers, applied in the directions of microorganisms, fertilizers made from biological waste, and methods based on microorganisms, can solve the problems of unsatisfactory disease prevention and production increase of microbial fertilizers, and achieve prevention and improvement. Effects of soil-borne diseases, increasing soil organic matter, and balanced nutrition
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Embodiment 1
[0018] The preparation of embodiment 1 composite microbial bacterial agent
[0019] This example describes the preparation method of the composite microbial agent that needs to be used in the preparation process of microbial fertilizer.
[0020] (1) Preparation of Bacillus subtilis (Bacillus subtilis) XF-1 bacterial agent
[0021] Activation of Bacillus subtilis (Bacillus subtilis) XF-1 (hereinafter abbreviated as Bacillus subtilis XF-1): From the slant of Bacillus subtilis XF-1, use a sterile inoculation needle to pick part of the lawn into the LB shake flask (50ml of LB liquid medium in a 250mL shake flask), after culturing at 30°C and 150r / min for 24h, the LB medium became turbid. Take the bacterial liquid and streak it into LB solid medium for 24h-36h. Bacillus subtilis XF-1 grows well on LB solid medium. The colonies are white or off-white, with smooth edges, irregular surface and opaque.
[0022] Preparation of seed solution: the liquid volume of LB liquid medium in th...
Embodiment 2
[0037] The preparation of embodiment 2 microbial fertilizer
[0038] Adopt composite microbial bacterial agent A to prepare microbial bacterial fertilizer, comprise the steps:
[0039] Step 1: Ferment in the pigsty to obtain a fermentation bed rich in beneficial microorganisms, organic matter and a large amount of active substances.
[0040] Take 500 parts by weight of rice husk, 200 parts by weight of brewer's grains, 100 parts by weight of sawdust, 100 parts by weight of soybean oil dregs, 50 parts by weight of leaves, 50 parts by weight of edible fungus dregs, 50 parts by weight of weeds, and 200 parts by weight of corn stalks, and mix evenly. Obtain the fermentation bed matrix. Among them, brewer's grains are the remaining solids after beer brewing, soybean oil residues are the solid residues in the cold filtration process of soybean oil produced by pressing process, and edible fungus residues are the waste solids left after various mushrooms are cultivated.
[0041] Ins...
Embodiment 3
[0056] The field effect of embodiment 3 microbial fertilizer
[0057] Using the microbial fertilizer prepared in Example 2, a large number of tests were carried out in Yunnan Province.
[0058] Application of crops and prevention / control of diseases: tomato bacterial wilt, cabbage clubroot, wheat root rot, cucumber blight, pepper blight, watermelon wilt, peanut root-knot nematode.
[0059] Fertilizer plan:
[0060] Select six fertilizer schemes for comparison: CK (no fertilizer), commercially available general organic fertilizer (General Organic Fertilizer, abbreviated as: GOF for labeling convenience), microbial fertilizer A, control microbial fertilizer 1 (abbreviated as D1) , control microbial bacterial fertilizer 2 (abbreviated as D2), and control microbial bacterial fertilizer 3 (abbreviated as D3). Microbial fertilizers A, D1, D2, and D3 were all prepared by the method in Example 2.
[0061] Usage and dosage: Fertilize 3 times during the cultivation of each plant, the...
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