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A kind of tannin and saponin degrading microbial bacterial agent

A technology for microbial inoculants and microbial strains is applied in the field of microorganisms to achieve the effects of easy cultivation, good degradation effect and low cost

Active Publication Date: 2021-04-20
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, few studies have reported the strains capable of simultaneously degrading both tannins and saponins

Method used

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  • A kind of tannin and saponin degrading microbial bacterial agent

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Embodiment Construction

[0019] The following specific examples are further descriptions of the methods and technical solutions provided by the present invention, but should not be construed as limiting the present invention.

[0020] (1) Screening, cultivation and preservation of bacterial strains:

[0021] 1. LB medium

[0022] Tryptone 10g, yeast extract powder 5g, sodium chloride 10g, and agar 15.0g, pH 7.

[0023] 2. Cultivation of microbial agents

[0024] A tannin- and saponin-degrading microbial agent obtained by screening natural rotten camellia husks, named Klebsiella pneumoniae 15440, preservation number: CGMCC NO.15440, preservation date: 2018 On March 9th, the preservation unit: General Microbiology Center of China Microbiological Culture Collection Management Committee, put the strain into the prepared LB broth medium at 30°C, shake at 150r / min for 3 days; then prepare the slant of the LB agar medium test tube , after solidification, the strains were inserted into the slant of the tes...

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Abstract

The invention discloses a microbial bacterial agent capable of simultaneously degrading tannin and saponin, and the microbial agent is screened from naturally decayed camellia oleifera husks. The microbial agent of the invention has the ability to degrade tannin and saponin, especially for plant materials rich in tannin and saponin, the degradation rate of tannin and saponin is greater than 50%, and the degradation effect is good.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a tannin and saponin degrading microbial bacterial agent. Background technique [0002] Tea saponin (TS) is a general term for a class of oleanane-type pentacyclic triterpenoid saponin structural analogues, mainly derived from the roots, leaves, flowers and Fruit, its monomer substance is often called tea saponin, tea saponin. Among them, Camellia oleifera saponin is a kind of natural glycoside compound contained in Camellia oleifera woody oil plant, which can be widely used in the fields of daily chemicals, building materials, textiles, pesticides and medicines, and has high economic value. Tea saponin mainly exists in camellia oleifera meal and camellia oleifera husk. Studies have shown that the content of tea saponin in camellia oleifera husk is about 5%, and the content of tea saponin in camellia oleifera meal is about 15%. Most of them are abandoned, and only some r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/22
CPCC05F11/08C12N1/20C12N1/205C12R2001/22
Inventor 张金萍李雪彬
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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