Bacillus altitudinis and application thereof in control of tomato root-knot nematode

A technology of Bacillus highlandi and tomato root-knot nematode, which is applied in the directions of application, pest control, nematicide, etc., can solve the problem of less Bacillus highlandi, and achieves high spore production, growth promotion effect, and good control effect. Effect

Inactive Publication Date: 2018-11-23
毕乃亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Bacillus altitudinis and application thereof in control of tomato root-knot nematode
  • Bacillus altitudinis and application thereof in control of tomato root-knot nematode
  • Bacillus altitudinis and application thereof in control of tomato root-knot nematode

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Embodiment 1

[0028] 1. Isolation and purification of Bacillus altitudinis YT2002

[0029] Bacillus altitudinis (B.altitudinis) YT2002 of the present invention adopts dilution plate method and plate streaking method to separate and obtain from the beach silt of Yangma Island, Muping, Yantai City, and the separation method is:

[0030] (1) Isolation of strains

[0031] The samples were collected from the beach sediment of Yangma Island, Muping, Yantai City on April 30, 2014. Weigh 1g of sediment sample into 100mL of sterile water, place it in a shaker at 30°C at 150rpm for 10min, then place it in a water bath at 60°C for 30min, take 100μL of 10 -2 、10 -3 、10 -4 The diluted solution is spread on the LB medium plate, and each gradient is coated in three parallels. After culturing at 30°C for 2 days, the microbial colonies of different forms on the LB medium are picked and streaked on the LB medium plate, and observed regularly Colony growth. Then, the bacillus strains were purified by str...

Embodiment 2

[0054] 1. Fermentation process of Bacillus highlander

[0055] The formula of LB liquid medium is: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, pH adjusted to 6.8-7.0.

[0056] Fermentation medium is made up of solid material and inorganic salt, and wherein solid material is: by mass ratio rice husk: corn flour: soybean meal flour: bran=50:24:20:6; The component and mass ratio of inorganic salt are: phosphoric acid Potassium dihydrogen 11%, magnesium sulfate 9%, ammonium sulfate 15%, light calcium carbonate 65%; The mass ratio of solid material and inorganic salt is 98.7: 0.3 (mass ratio). Preparation method: adding an appropriate amount of water (10-15 times the quality) to dissolve the inorganic salt to obtain an inorganic salt solution; mixing the inorganic salt solution with the solid material, and adding water to finally adjust the water content of the solid medium to 40-60%, so that the Hold the solid medium in your hand, and it is advisa...

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Abstract

The invention discloses a strain of bacillus altitudinis and application thereof in control of tomato root-knot nematode. The bacillus altitudinis strain YT2002 has been deposited in the China GeneralMicrobiological Culture Collection Center (CGMCC) in January 15, 2018 with the Accession No. of CGMCC No. 15213; experimental results show that the bacillus altitudinis strain YT2002 biological agenthas a good effect on control of tomato root-knot nematode, with the control effect of 63.03% after 14 days after the last application; from growth of the tomato, the bacillus altitudinis strain YT2002 has an obvious effect on promoting tomato growth, and is safe and reliable without risk of damage.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control. Specifically, the present invention relates to a highland bacillus strain and its application in controlling root-knot nematode of tomato, and also relates to a biological control preparation prepared by using the highlander bacillus. Background technique [0002] Root-knot nematode (Meloidogyne spp.) is one of the main diseases of tomato, which is widely distributed all over the world. With the increase of vegetable production area in protected areas, especially since the large-scale promotion of solar greenhouses, the multi-cropping index has increased, and the heavy cropping has caused the damage of root-knot nematodes to become more and more serious. Generally, it can cause a 10% to 20% reduction in production, and it can reach 30% in severe cases. % to 40%, or even extinct. The harm of root-knot nematodes to tomato is manifested in the fact that when the root-knot ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P5/00A01P21/00A01G13/00C12R1/07
CPCA01G13/00A01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 毕乃亮牛赡光刘宜鑫张淑静牛兆颖李宗泰
Owner 毕乃亮
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