Fluorescence enhancement type hypochlorite detection method and application
A technology for detecting hypochlorous acid and fluorescence enhancement, which is applied in the fields of fluorescence/phosphorescence, measuring devices, and material analysis through optical means. It can solve the problems of difficult detection of hypochlorite ions and low detection sensitivity, and increase the scope of practical applications. , Strong anti-interference ability and high anti-interference effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0036] 1. Preparation of fluorescent probe SRh
[0037] 1. Preparation of RhB-SH
[0038] In a 100 mL round bottom flask, dissolve 2.18 g (4.5 mmol) of rhodamine B in 30 mL of anhydrous 1,2-dichloroethane, and add 1.5 mL (16.4 mmol) of phosphorus oxychloride dropwise under ice-cooling conditions , react in ice bath (0°C) for 1 hour, change to reflux reaction at 85°C for 3 hours, remove the solvent under reduced pressure to obtain rhodamine acid chloride RhB-Cl; dissolve rhodamine acid chloride RhB-Cl in 30 mL of anhydrous acetonitrile, Add 0.51 g (4.5 mmol) cysteamine hydrochloride in anhydrous acetonitrile solution (15 mL) dropwise under ice bath (0°C) condition, react in ice bath for 6 hours, warm the reaction system to room temperature, remove the solvent under reduced pressure, and use Ethyl acetate (35 mL) was redissolved, washed with pure water (40 mL×2) and saturated sodium chloride solution (40 mL×2), the organic phase was dried over anhydrous sodium sulfate, filtered...
Embodiment 1
[0046] Prepare a Tris buffer solution with a pH of 7.4 and a concentration of 10 mM, use the above buffer solution and ethanol to prepare a mixed solution A with a volume ratio of 7:3, and use ethanol to prepare a probe SRh solution with a concentration of 1 mM. The effect of probe SRh on hypochlorite (ClO - ) in the selectivity of mixed solution A. Such as figure 1 As shown, under the excitation condition at 525 nm, the single probe SRh (10 µM) has a weak fluorescence emission intensity at 585 nm in mixed solution A, when adding hypochlorite (ClO - ) (10 eq.), the fluorescence emission intensity at 585 nm was significantly enhanced, but when other substances (100 µM) were added, the fluorescence emission intensity of the solution system did not change significantly compared with that of the probe system alone.
[0047] The above experimental results show that the probe SRh is sensitive to hypochlorite (ClO - ) has better fluorescence-specific selectivity in mixed solution...
Embodiment 2
[0049] Prepare a Tris buffer solution with a pH of 7.4 and a concentration of 10 mM, use the above buffer solution and ethanol to prepare a mixed solution A with a volume ratio of 7:3, and use ethanol to prepare a probe SRh solution with a concentration of 1 mM. In 20 clean fluorescent cuvettes, add 3000 μL of mixed solution A and 30 μL of probe SRh ethanol solution, and then add 10 molar equivalents of ClO - and 10 molar equivalents of other analytes (Br - , Cl - , F - , I - , CO 3 2- , HCO 3 - , PO 4 3- , HPO 4 2- , H 2 PO 4 - ,HS - , NO 2 - , NO 3 - , MnO 4 - , SO 4 2- , H 2 o 2 , KO 2 , TBHP, Cys, GSH) were detected on a fluorescence spectrometer, and the histograms of the 585 nm fluorescence intensities corresponding to different analytes were drawn to obtain the fluorescence emission histograms (attached figure 2 ).
[0050] It has been proved by experiments that the probe SRh is sensitive to hypochlorite (ClO - ) in the mixed solution A is n...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More