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Applications of chitosan-se in inhibiting lowering of total antioxidant capacity of swine endometrial epithelium cells caused by F-2 toxin

A total antioxidant capacity, endometrium technology, applied in epidermal cells/skin cells, animal cells, reproductive tract cells, etc., can solve the problem of no chitosan selenium, no anti-oxidation of endometrial epithelial cells. capacity, reduction, etc.

Inactive Publication Date: 2018-11-30
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no patents or reports on chitosan selenium in alleviating the toxic effect of F-2 toxin on endometrial epithelial cells at home and abroad. Patents or reports on the reduction of total antioxidant capacity of epithelial cells

Method used

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  • Applications of chitosan-se in inhibiting lowering of total antioxidant capacity of swine endometrial epithelium cells caused by F-2 toxin
  • Applications of chitosan-se in inhibiting lowering of total antioxidant capacity of swine endometrial epithelium cells caused by F-2 toxin
  • Applications of chitosan-se in inhibiting lowering of total antioxidant capacity of swine endometrial epithelium cells caused by F-2 toxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1.1 Isolation and purification of porcine endometrial cells

[0021] (1) Isolation of endometrial cells (endometrial epithelial cells were isolated by tissue block method)

[0022] Immediately after the slaughter of the pig, the two ends of the uterus were ligated, and the whole uterus was aseptically removed, the fat tissue was peeled off, rinsed with 200 IU double-antibody pre-cooled normal saline solution, placed in the double-antibody-containing normal saline solution, and sent to the hospital as soon as possible. to the lab. Clamp both ends of the uterus with hemostatic forceps, take out the uterus in the ultra-clean workbench, soak it in alcohol for about 60 seconds, wash it with PBS after taking it out; cut the uterus longitudinally (close to the uterine horn), wash it 3-4 times with double-antibody PBS, without Bloodstain, cut endometrium to 1 mm 3 Left and right granular, placed in the cell culture plate evenly at intervals of about 0.5 cm, after the tissue b...

Embodiment 2

[0038] 2.1 Isolation and purification of porcine endometrial cells

[0039] (1) Isolation of endometrial cells (endometrial epithelial cells were isolated by tissue block method)

[0040] Immediately after the slaughter of the pig, the two ends of the uterus were ligated, and the whole uterus was aseptically removed, the fat tissue was peeled off, rinsed with 200 IU double-antibody pre-cooled normal saline solution, placed in the double-antibody-containing normal saline solution, and sent to the hospital as soon as possible. to the lab. Clamp both ends of the uterus with hemostatic forceps, take out the uterus in the ultra-clean workbench, soak it in alcohol for about 60 seconds, wash it with PBS after taking it out; cut the uterus longitudinally (close to the uterine horn), wash it 3-4 times with double-antibody PBS, without Bloodstain, cut endometrium to 1 mm 3 Left and right granular, placed in the cell culture plate evenly at intervals of about 0.5 cm, after the tissue b...

Embodiment 3

[0056] 3.1 Isolation and purification of porcine endometrial cells

[0057] (1) Isolation of endometrial cells (endometrial epithelial cells were isolated by tissue block method)

[0058] Immediately after the slaughter of the pig, the two ends of the uterus were ligated, and the whole uterus was aseptically removed, the fat tissue was peeled off, rinsed with 200 IU double-antibody pre-cooled normal saline solution, placed in the double-antibody-containing normal saline solution, and sent to the hospital as soon as possible. to the lab. Clamp both ends of the uterus with hemostatic forceps, take out the uterus in the ultra-clean workbench, soak it in alcohol for about 60 seconds, wash it with PBS after taking it out; cut the uterus longitudinally (close to the uterine horn), wash it 3-4 times with double-antibody PBS, without Bloodstain, cut endometrium to 1 mm 3 Left and right granular, placed in the cell culture plate evenly at intervals of about 0.5 cm, after the tissue b...

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Abstract

The invention discloses applications of chitosan-se in inhibiting lowering of the total antioxidant capacity of swine endometrial epithelium cells caused by F-2 toxin. The experiment result shows thatfor the endometrial epithelium cells, 0.8-3.0 [mu] mol / L chitosan-se (the final concentration in a culture solution is calculated in terms of se) is used, when the concentration of the F-2 toxin is within the range of 10-30 [mu] g / mL, the lowering of the total antioxidant capacity of the swine endometrial epithelium cells caused by the F-2 toxin can be improved, the toxic effects of the F-2 toxinfor the swine endometrial epithelium cells cultured in vitro can be weakened, and thus an in-vitro study model and theoretical support are provided for the applications of chitosan-se in preventing and treating swine poisoning caused by the F-2 toxin.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of chitosan selenium in inhibiting the reduction of the total antioxidant capacity of porcine endometrium epithelial cells caused by F-2 toxin. Background technique [0002] F-2 toxin, also known as zearalenone, is a mycotoxin produced by the genus Fusarium. It is a dihydroxybenzoic acid lactone compound. It has a similar structure to estradiol and has estrogen-like activity. It is widely present in molds. It is one of the main mycotoxins in food and feed contamination. A large number of studies at home and abroad have proved that F-2 toxin has reproductive toxicity, cytotoxicity, liver and kidney toxicity, immunotoxicity and genotoxicity. Long-term feeding of feed contaminated with F-2 toxin will lead to decreased growth performance, low immune function and reproductive dysfunction of livestock and poultry. [0003] Studies have shown that oxidative damage is one o...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0625C12N5/0682C12N2500/34C12N2500/10
Inventor 秦顺义王欢欢张建斌马吉飞杨升李留安金天明李存刘田生
Owner TIANJIN AGRICULTURE COLLEGE