Preparation method of chitosan molecular beacon nanocomposite

Abstract

The invention provides a preparation method of a chitosan molecular beacon nanocomposite. The chitosan miR-155 (microRNA-155) molecular beacon nanocomposite is synthesized by a self-assembly method. The chitosan miR-155 (microRNA-155) molecular beacon nanocomposite can dynamically monitor the occurrence and development processes of lung cancer according to the fluorescence intensity. At the same time, the expression of miR-155 can be further inhibited by combination with the miR-155 highly expressed in cancer cells, so as to achieve the purpose of inhibiting tumor; the nanocomposite can be applied in living animal imaging and tumor therapy of lung cancer cells and lung cancer mice with tumor, has the characteristics of high transfection efficiency, high specificity, good stability, low background and high inhibition rate, can provide new ideas, new methods and new technologies for early-stage diagnosis and treatment of lung cancer.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method of a chitosan molecular beacon nanocomposite. Background technique [0002] Lung cancer is a malignant tumor that seriously threatens human health. Its morbidity and mortality are increasing year by year. Early diagnosis and early treatment are the key to improving the prognosis of patients. However, lung cancer patients often have no obvious symptoms in the early stage of the disease. best time for treatment. Therefore, starting from the "origin" of lung cancer, exploring new methods for efficient and specific early diagnosis and treatment will have a profound impact on the prevention, diagnosis and treatment of lung cancer. [0003] The theory of cancer stem cells holds that there is a group of very small number of tumor cells in tumor tissue cells, called tumor stem cells, and all tumor cells are differentiated from tumor stem cells. MicroRNA (miRNA,...

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Problems solved by technology

[0006] In order to overcome the deficiencies in the prior art, the purpose of the present invention is to provide a preparation method of chitosan molecular beacon nanocomposites, which solves the complex preparation method of drugs or reagents used for lung cancer gene diagnosis and treatment in the prior art. , harsh process conditions, poor specificity or stability, and the problem that it can only diagnose intermediate and advanced lung cancer