Preparation method of chitosan molecular beacon nanocomposite

A chitosan nano- and nano-composite technology, which is applied in the field of biomedicine, can solve problems such as harsh process conditions, complicated preparation methods, poor specificity or stability, and achieve high transfection efficiency, good stability, and specificity. sex high effect

Inactive Publication Date: 2018-12-07
GUIZHOU PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the deficiencies in the prior art, the purpose of the present invention is to provide a preparation method of chitosan molecular beacon nanocomposites, which solves the complex preparation method...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Mix chitosan nanometers and molecular beacons in PBS buffer at pH 6.0 in a mass ratio of 7:1, vortex for 60 seconds, and stand at room temperature for 60-120 minutes to obtain chitosan molecular beacon nanocomposites things.

Embodiment 2

[0020] Mix the chitosan nanometer and the molecular beacon in the PBS buffer of pH=6.5 according to the mass ratio of 8:1, vortex and shake for 60s, and stand at room temperature for 60-120min to obtain the chitosan molecular beacon nanometer Complex.

Embodiment 3

[0022] Mix the chitosan nanometer and the molecular beacon in the PBS buffer of pH=7.0 according to the mass ratio of 10:1, vortex and shake for 60s, and stand at room temperature for 60-120min to obtain the chitosan molecular beacon nanometer Complex.

[0023] In the above examples, chitosan nanometers and molecular beacons were mixed in PBS buffer solution with pH 6.0-7.0 according to the mass ratio of 7:1 to 10:1, vortexed for 40s to 120s, and left to stand at room temperature 40min-120min to obtain the chitosan molecular beacon nanocomposite. Any combination within this range will not affect the product and can be combined arbitrarily.

[0024] The following examples are related experiments in which chitosan molecular beacon nanocomposites are used for diagnosis and treatment of lung cancer.

[0025] Embodiment 2: liquid phase molecular hybridization experiment

[0026] Liquid-phase molecular hybridization experiments verified the correctness and specificity of the desi...

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Abstract

The invention provides a preparation method of a chitosan molecular beacon nanocomposite. The chitosan miR-155 (microRNA-155) molecular beacon nanocomposite is synthesized by a self-assembly method. The chitosan miR-155 (microRNA-155) molecular beacon nanocomposite can dynamically monitor the occurrence and development processes of lung cancer according to the fluorescence intensity. At the same time, the expression of miR-155 can be further inhibited by combination with the miR-155 highly expressed in cancer cells, so as to achieve the purpose of inhibiting tumor; the nanocomposite can be applied in living animal imaging and tumor therapy of lung cancer cells and lung cancer mice with tumor, has the characteristics of high transfection efficiency, high specificity, good stability, low background and high inhibition rate, can provide new ideas, new methods and new technologies for early-stage diagnosis and treatment of lung cancer.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method of a chitosan molecular beacon nanocomposite. Background technique [0002] Lung cancer is a malignant tumor that seriously threatens human health. Its morbidity and mortality are increasing year by year. Early diagnosis and early treatment are the key to improving the prognosis of patients. However, lung cancer patients often have no obvious symptoms in the early stage of the disease. best time for treatment. Therefore, starting from the "origin" of lung cancer, exploring new methods for efficient and specific early diagnosis and treatment will have a profound impact on the prevention, diagnosis and treatment of lung cancer. [0003] The theory of cancer stem cells holds that there is a group of very small number of tumor cells in tumor tissue cells, called tumor stem cells, and all tumor cells are differentiated from tumor stem cells. MicroRNA (miRNA,...

Claims

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Application Information

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IPC IPC(8): A61K47/36A61K49/00
CPCA61K47/36A61K49/003A61K49/0054
Inventor 朱海振郭熠朱海燕候净刘鑫胡建军江飞龙李杭谭诗生杨飞月俞婕崔媛媛
Owner GUIZHOU PROVINCIAL PEOPLES HOSPITAL
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