Antibacterial and healing-promoting silk protein wound-protecting material and preparation method thereof
The technology of fibroin and antibacterial agent is applied in the field of antibacterial and healing-promoting fibroin wound-protecting materials and the field of preparation thereof, which can solve the problems of single function, lack of comprehensive therapeutic effect of antibacterial and promoting healing, etc. Simple and reliable raw materials, good biodegradability
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[0024] In addition, the embodiment of the present invention also provides a preparation method of an antibacterial and healing-promoting silk protein wound-protecting material, which includes adding growth factor-loaded slow-release particles and antibacterial agent-loaded slow-release particles into the silk protein solution, mixing evenly to obtain Spinning stock solution, the spinning stock solution is made into a three-dimensional structure of antibacterial and healing-promoting silk protein wound protection material. It specifically includes the following steps:
[0025] S1. Preparation of slow-release microparticles loaded with growth factors.
[0026] drop the phosphate-buffered saline solution containing growth factors into the methylene chloride solution containing the first particulate carrier, centrifuge after stirring, discard the supernatant, add water to ultrasonically disperse and wash the phosphate-buffered saline solution, and freeze-dry to obtain the product;...
Embodiment 1
[0042] This example provides an antibacterial and healing-promoting silk protein wound-protecting material, whose components include a silk protein solution with a mass ratio of 40:1:1, slow-release particles loaded with growth factors, and slow-release particles loaded with antibacterial agents. Wherein, both the first particle carrier and the second particle carrier are PLGA. Growth factors include basic fibroblast growth factor, epidermal growth factor, and dermal growth factor; antibacterial agents include chitosan.
[0043] Its preparation method includes:
[0044] S1. Preparation of slow-release microparticles loaded with growth factors.
[0045] Add PLGA to the dichloromethane solution to dissolve to obtain a 10 mg / ml dichloromethane solution containing PLGA; add the phosphate-buffered saline solution containing growth factors at a concentration of 1 mg / ml into the above-mentioned PLGA-containing dichloromethane solution drop by drop at a uniform speed In, the mass ra...
Embodiment 2
[0057] This example provides an antibacterial and healing-promoting silk protein wound-protecting material, whose components include a silk protein solution with a mass ratio of 30:1:1, slow-release particles loaded with growth factors, and slow-release particles loaded with antibacterial agents.
[0058] Wherein, both the first particulate carrier and the second particulate carrier are PLA. Growth factors include basic fibroblast growth factor; antibacterial agents include chitosan, aloe vera, mugwort, juniper oil, antimicrobial peptides, and berberine.
[0059] Its preparation method includes:
[0060] S1. Preparation of slow-release microparticles loaded with growth factors.
[0061] PLA was added into the dichloromethane solution to dissolve to obtain a 5 mg / ml dichloromethane solution containing the first microparticle carrier; the phosphate-buffered saline solution containing the growth factor concentration of 2 mg / ml was dripped dropwise into the above-mentioned PLA-co...
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