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Reagent and method for diagnosing unstable plaque of therosclerosis

A technology of atherosclerosis and reagents, applied in the field of medical diagnosis, can solve the problems of lack of nuclear medicine probes for high-risk plaques, low signal-to-noise ratio, unstable clinical diagnosis, etc., achieving good application prospects, huge social benefits and The effect of economic benefits

Inactive Publication Date: 2018-12-11
KUNSHAN XINYUNDA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of nuclear medicine probes for high-risk plaques in clinical diagnosis. 18 F-FDG is the gold standard for the diagnosis of arterial plaques, but due to its high myocardial absorption, the signal-to-noise ratio of its detection is low, and the clinical diagnosis of unstable plaques is limited.

Method used

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  • Reagent and method for diagnosing unstable plaque of therosclerosis
  • Reagent and method for diagnosing unstable plaque of therosclerosis
  • Reagent and method for diagnosing unstable plaque of therosclerosis

Examples

Experimental program
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Effect test

Embodiment 199m

[0054] Example 1 99m Radiosynthesis of Tc-HFn

[0055] Expression and purification of H-ferritin: using the cDNA of the Hela cell line that highly expresses H-ferritin (also referred to as HFn in this paper) as a template, the full-length primers of human H-ferritin were designed, and the human H-ferritin The cDNA of 552bp was constructed on the expression vector pET30a (Novagen); then, using the prokaryotic expression system, the HFn-pET30a was transformed into the expression strain BL21(DE3) (Novagen), and the expression was induced by IPTG, and then the human HFn protein was purified (about For molecular construction, expression and purification of human H-ferritin, please refer to Nature Nanotech.2012 and Chinese Invention Patent Application 201110122433.0).

[0056] The nucleotide coding sequence of H-ferritin is as follows:

[0057] ATGACGACCGCGTCCACCTCGCAGGTGCGCCAGAACTACCACCAGGACTCAGAGGCCGCCATCAACCGCCAGATCAACCTGGAGCTCTACGCCTCCTACGTTTACCTGTCCATGTCTTACTACTTTGACCGCGATGAT...

Embodiment 299m

[0059] Example 2 99m Study on the Detection of Unstable Plaques by Tc-HFn

[0060] Apoe- / - mice (purchased from the Experimental Animal Center of Peking University Health Science Center) purchased 8 mice in each group, carried out high-fat diet (containing 10% fat, 2% cholesterol and 0.5% sodium cholate), and fed them for 12 weeks and 20 years respectively. At week 1 and week 33, atherosclerotic plaques of different sizes were formed for imaging detection (experimental group), figure 2 a The arterial plaques formed in mice fed with high fat for different periods were identified by Oil Red O staining, figure 2 b The plaque area of ​​mice with atherosclerotic plaque was quantified. Wild-type C57 healthy mice were used as disease controls, and wild-type mice had no arterial plaque formation ( figure 2 ). The mice in the experimental group and the control group were intravenously injected with a dose of 500 μCi 99m Tc-HFn diagnostic reagent, SPECT / CT imaging was performed ...

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Abstract

The invention provides a reagent and a method for diagnosing unstable plaque of therosclerosis. Specifically, a detection marker for a protein core capable of particularly identifying unstable plaqueof therosclerosis is used for marking, so as to realize the in vivo flexible detection for unstable plaque. The invention also provides the method for detecting the unstable plaque on the basis of thediagnosis reagent.

Description

field of invention [0001] The invention belongs to the field of medical diagnosis, and in particular relates to a radiodiagnostic technique using nanometer materials, in particular to a method for diagnosing unstable plaques of atherosclerosis. Background technique [0002] At present, cardiovascular and cerebrovascular diseases have surpassed cancer and ranked first among the deadly diseases [1]. According to reports, in the United States, about 800,000 people die of cardiovascular and cerebrovascular diseases every year, and an average of about 6 deaths are related to cardiovascular and cerebrovascular diseases [1]. Atherosclerosis is the most common and most important inducing and lethal factor of cardiovascular and cerebrovascular diseases [2]. In the past, it was believed that acute heart disease events caused by atherosclerosis were mainly caused by coronary stenosis; however, in recent years, studies have found that angiography in patients with acute myocardial infar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K51/08A61K49/04A61K101/02A61K103/10A61K103/20A61K103/00
CPCA61K49/04A61K51/08
Inventor 阎锡蕴梁敏敏程登峰谭辉石洪成
Owner KUNSHAN XINYUNDA BIOTECH CO LTD