Method for detecting genistein and metabolites thereof in biological sample

A technology of biological samples and detection methods, applied in the direction of measuring devices, material separation, and analysis of materials, can solve the problems of no detection method for genistein metabolites and the inability to determine the metabolic process of genistein

Inactive Publication Date: 2018-12-11
北京鸿测科技发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there is no report on the detection method of genistein metabolites, and its metabolit...

Method used

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  • Method for detecting genistein and metabolites thereof in biological sample
  • Method for detecting genistein and metabolites thereof in biological sample
  • Method for detecting genistein and metabolites thereof in biological sample

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Experimental program
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Embodiment 1

[0053] 1 Experimental instruments and materials

[0054] DIONEX Ultimate 3000 ultra-high performance liquid chromatography analysis system: American Thermo Fisher Company;

[0055] LTQ-Orbitrap XL mass spectrometer: American Thermo Fisher Company, equipped with electrospray ion source (ESI) and Xcalibur 2.1 workstation;

[0056] Grace Pure TM SPEC 18 -Low SPE cartridge (500mg / 3mL);

[0057] Milli-Q Synthesis ultrapure water purification system: Millipore, USA;

[0058] R200D electronic analytical balance (1 / 100,000): German Sartorius company;

[0059] KQ-250DE CNC ultrasonic cleaner: Kunshan Ultrasonic Instrument Co., Ltd.

[0060] Genistein reference substance: product of Chengdu Master Biological Technology Co., Ltd. (purity greater than 98%);

[0061] Formic acid (chromatographically pure): Germany Merck company;

[0062] Methanol (mass spec pure) and acetonitrile (mass spec pure): American Thermo Fisher Company.

[0063] Sprague Dawley (SD) rats (male, weighing 220...

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Abstract

The invention discloses a method for detecting genistein and metabolites thereof in a biological sample, which is characterized by comprising the following steps: (1) processing the biological sample;and (2) detecting a to-be-detected solution by an ultra high performance liquid chromatography-mass spectrometer. The detection method disclosed by the invention can detect more genistein metabolitesin a biological sample, thereby laying a foundation for clarifying the metabolic pathways of genistein.

Description

technical field [0001] The invention relates to a detection method for genistein and its metabolites in biological samples. Background technique [0002] Genistein (GS), also known as 5,7,4′-trihydroxyisoflavone, genistein, genistein, genistein, exists in various edible plants, especially in kudzu root , alfalfa, oats, sophora, wheat, barley and broad bean root content is higher. As a natural tyrosine kinase with high activity and function, genistein is called "phytoestrogen" because of its structure similar to estrogen. In recent years, studies have found that genistein also has anti-oxidation, blood lipid-lowering, anti-tumor and osteoporosis prevention and other activities. At present, genistein has been widely used in medicine, health care products and other fields. [0003] At present, there is no report on the detection method of genistein metabolites, and no report on the components of its metabolites, so the metabolic process of genistein in vivo cannot be determi...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/065
Inventor 张连中张加余王志斌
Owner 北京鸿测科技发展有限公司
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