Fluorescent molecular marker of rice grain width regulatory gene GW8 and primers thereof

A fluorescent molecule and gene regulation technology, applied in the field of biomolecules, can solve the problems of using toxic substances, low efficiency, etc., and achieve the effects of rapid inspection, simple and reliable method, and improvement of grain shape agronomic traits

Active Publication Date: 2018-12-14
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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Problems solved by technology

[0004] Aiming at the above technical problems, the present invention discloses a fluorescent molecular marker of the rice grain width regulating gene GW8 and its primers, which realizes rapid detection of whet

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  • Fluorescent molecular marker of rice grain width regulatory gene GW8 and primers thereof
  • Fluorescent molecular marker of rice grain width regulatory gene GW8 and primers thereof

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[0026] The preferred embodiments of the present invention will be described in further detail below.

[0027] A fluorescent molecular marker for rice grain width regulation gene GW8, which adopts the following steps:

[0028] (1) Take rice leaves and obtain rice genomic DNA by CTAB extraction method (hexadecyl trimethyl ammonium bromide method);

[0029] (2) PCR amplification: adding three primers Allele-AT, Allele-GC and Common-R1 into the PCR reaction system at the same time, and amplifying the GW8 gene in the rice plant to obtain a PCR product.

[0030] The primer sequence is as follows:

[0031] Allele-AT: GAAGGTCGGAGTCAACGGATTACTAATTTGTGCTGATATATCAAACATCAT;

[0032] Allele-GC: GAAGGTGACCAAGTTCATGCTACTAATTTGTGCTGATATATCAAACATCGC;

[0033] Common-R1: TGAGAATCTTGCCCCTGAATTTTG.

[0034] Among them, the PCR reaction program is: 95°C, 5min; then 10 cycles of 95°C, 20sec, 65°C (-0.8°C per cycle), 1min; then 32 cycles of 95°C, 20sec, 57°C, 1min;

[0035] The PCR reaction system is 10ul: 5μL 2×...

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Abstract

The invention provides a fluorescent molecular marker of a rice grain width regulatory gene GW8 and primers thereof. The primers include an Allele-AT primer, an Allele-GC primer and a Common-R1 primer. The Allele-AT primer has the sequence as shown in SEQ ID NO:1, the Allele-GC primer has the sequence as shown in SEQ ID NO:2, and the Common-R1 primer has the sequence as shown in SEQ ID NO: 3. By adopting of the technical scheme of the invention, whether the rice variety contains a GW8 allele with deletion of two bases can be rapidly detected; the method is simple, and the shortcomings of low efficiency and use of toxic substances in the used method at present are solved; the fluorescent molecular marker can be applied to molecular marker-assisted selection of the GW8 allele and improves grain-shape agronomic traits of rice.

Description

technical field [0001] The invention belongs to the technical field of biomolecules, and in particular relates to a fluorescent molecular marker of rice grain width regulating gene GW8 and a primer thereof. Background technique [0002] As one of the important food crops, rice feeds about 50% of the world's population. Simultaneously improving rice yield and quality remains a challenging problem for rice breeders since rice yield and quality are negatively correlated. Grain size is an important factor affecting yield and quality, and is one of the main goals of rice breeding. In high-temperature areas, grain filling is difficult due to wide grain size, resulting in a decline in rice quality. However, the loss of function of the grain width negative regulator gene GW8 resulted in thinner grains and improved rice quality. Therefore, the development of fluorescent molecular markers of GW8 gene for molecular marker-assisted selection breeding has important research significan...

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 邓国富高利军卿冬进戴高兴黄娟高菊梁海福伍豪周维永周萌陈韦韦
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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