Continuous preparation method for antibody (or antigen)-coated microspheres

Abstract

The invention discloses a continuous preparation method for antibody (or antigen)-coated microspheres. The method comprises the specific preparation steps that first, microspheres, an antibody or antigen solution, a condensing agent and a buffer solution are prepared; and second, the microspheres in a microsphere tank and the condensing agent in a condensing agent tank are injected into a first tank body through a first pressure pump and a pipeline for mixing, and the antibody or antigen solution in an antibody or antigen solution tank and the buffer solution in a buffer solution tank are injected into a second tank body through the first pressure pump and the pipeline for mixing. According to the method, by introducing hot air through air inlet pipes in the liquid mixing process, liquid mixing is more uniform, and mixed liquid can be heated to improve the speed and efficiency of microsphere coating by antibodies or antigens, so that the microspheres are better coated; and moreover, the processing steps are simple, continuous preparation can be realized, and the method can be suitable for large-batch production.

Description

Technical field [0001] The present invention relates to the field of antibody (or antigen) coated microspheres, in particular to a continuous preparation method of antibody (or antigen) coated microspheres. Background technique [0002] The process of coating (adsorbing) antibodies or antigens onto microspheres can be divided into two categories. One is called the physical adsorption method. The basic process is to mix the antibody or antigen with the microspheres to be coated in a suitable buffer system, and use the interaction force between the antibody or antigen and the surface of the microspheres to produce adsorption. The molecules are fixed on the surface of the microspheres to realize the process of coating the microspheres. Another method is called chemisorption. The basic process is to use some active groups (such as carboxyl, amino, hydroxyl, sulfhydryl, etc.) on the surface of the microspheres and the corresponding groups (amino, carboxyl, sulfhydryl) on macromolecul...

AI Technical Summary

Problems solved by technology

Under such operating conditions, completely uniform mixing between the two phases is not easy to achieve, and the microenvironment around each microsphere may vary greatly, which directly leads to the difference between the coated microspheres. Large, large product differences between batches of coating reaction production, low utilization rate of antibodies (or antigens), etc.

And before mixing the microspheres and the antibody (or antigen), the microspheres and the antibody (or antigen) should be dispersed very evenly, otherwise the microspheres are prone to agglomeration after the antibody is labeled, resulting in uneven coating of the microspheres

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