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Method for preparing sulforaphane microcapsules by means of emulsification and spray drying method

A spray drying method, sulforaphane technology, applied in food drying, application, food forming and other directions, can solve the problem of poor stability improvement effect of sulforaphane, high toxicity of curing agent formaldehyde, difficult operation process, etc. problems, to achieve the effect of solving stability problems, uniform size, and simple equipment

Active Publication Date: 2018-12-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it only disperses sulforaphane in the capsule material and sprays it dry directly. The amount of capsule material used is large, and the effect of improving the stability of sulforaphane is not good.
In CN107569470A, jelly jelly and gelatin with good physical properties are used as capsule materials, and sulforaphane microcapsules are prepared by composite coacervation method. This process is sensitive to temperature and pH, and the operation process is difficult, and the curing agent formaldehyde used is highly toxic

Method used

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  • Method for preparing sulforaphane microcapsules by means of emulsification and spray drying method
  • Method for preparing sulforaphane microcapsules by means of emulsification and spray drying method
  • Method for preparing sulforaphane microcapsules by means of emulsification and spray drying method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Accurately weigh 2.0 kg of broccoli seeds, put them into a crusher to crush, sieve to a mesh number of 40 mesh, and feed broccoli seed powder to supercritical CO 2 In the extraction kettle, control the extraction pressure to 30MPa, the extraction temperature to 40°C, the extraction time to 4h, CO 2 The flow rate is 7BV / h, and broccoli seed defatted powder is obtained by separation; the defatted powder is added to 10BV water, and endogenous enzymes are used to hydrolyze to obtain the sulforaphane extract; The volume ratio between the element extract and DM850 resin is 1:22ml, the ratio of diameter to height of the resin column is 1:8, and the flow rate is 4BV / h to pass through the resin column. % ethanol to remove some impurities, then elute with 6BV of 50% ethanol, collect the eluate and concentrate in vacuo; after concentrating to no alcohol smell, add 2BV of ethyl acetate to extract twice, and concentrate in vacuo to remove the organic solvent.

[0031] (2) Take ...

Embodiment 2

[0035] (1) Accurately weigh 2.0 kg of broccoli seeds, put them into a crusher and crush them, sieve to a mesh number of 60 mesh, and feed broccoli seed powder to supercritical CO 2 In the extraction kettle, control the extraction pressure to 25MPa, the extraction temperature to 40°C, the extraction time to 2h, CO 2 The flow rate is 8BV / h, and broccoli seed defatted powder is obtained by separation; the defatted powder is added to 10BV water, and endogenous enzymes are used to hydrolyze to obtain the sulforaphane extract; The ratio of prime extract to SP850 resin is 1:22ml by volume, the diameter-to-height ratio of the resin column is 1:8, and the flow rate is 4BV / h to pass through the resin column. % ethanol to remove some impurities, then elute with 6BV of 50% ethanol, collect the eluate and concentrate in vacuo; after concentrating until there is no alcohol smell, add 2BV of dichloromethane to extract twice, and concentrate in vacuo to remove the organic solvent.

[0036](2...

Embodiment 3

[0040] (1) Accurately weigh 2.0 kg of broccoli seeds, put them into a crusher and crush them, sieve to a mesh number of 20 mesh, and feed broccoli seed powder to supercritical CO 2 In the extraction kettle, control the extraction pressure to 40MPa, the extraction temperature to 30°C, the extraction time to 4h, CO 2 The flow rate is 6BV / h, and broccoli seed defatted powder is obtained by separation; the defatted powder is added to 10BV water, and endogenous enzymes are used to hydrolyze to obtain the sulforaphane extract; The volume ratio between the element extract and DM850 resin is 1:22ml, the ratio of diameter to height of the resin column is 1:8, and the flow rate is 4BV / h to pass through the resin column. % ethanol to remove some impurities, then elute with 6BV of 50% ethanol, collect the eluate and concentrate in vacuo; after concentrating until there is no alcohol smell, add 2BV of n-butanol to extract twice, and concentrate in vacuo to remove the organic solvent.

[0...

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Abstract

The invention discloses a method for preparing sulforaphane microcapsules by means of an emulsification and spray drying method. Broccoli seeds are used as a raw material, after being crushed, the broccoli seeds are degreased through supercritical treatment, an endogenous enzyme is used for hydrolysis to extract sulforaphane, then by means of macroporous resin and an organic solvent extraction method, the sulforaphane is purified, then an emulsifier, an antioxidant and oil are added into the sulforaphane to prepare an oil phase, polyethylene glycol and water are added into a compound capsule wall material to prepare a water phase, according to a core material ratio, the oil phase is added into the water phase while stirring, high-speed shearing is conducted, and finally, obtained emulsionis subjected to spray drying to prepare the sulforaphane microcapsules. The prepared sulforaphane microcapsules are even in size and smooth in surface; the embedding rate reaches 80.95%, and the microcapsule grain size is 1.03-3.38 mum; under a condition of a 90-DEG C high temperature, the sulforaphane microcapsules are placed for 5 h, and the sulforaphane retention rate can reach 90% or above. The method is simple in process and low in production cost, and the high temperature tolerance of the sulforaphane can be improved, so that the method for preparing the sulforaphane microcapsule by means of the emulsification and spray drying method is suitable for industrialized application.

Description

technical field [0001] The invention belongs to the field of food, and relates to a method for preparing sulforaphane microcapsules by using an emulsification spray drying method. Background technique [0002] Sulforaphane [1-isothiocyanate (4R)-4-(methylsulfinyl) butane, Sulforaphane] is the hydrolysis product of glucoraphanin, a yellow oily liquid, easily soluble in methanol, acetonitrile, acetic acid In organic solvents such as ethyl ester. Sulforaphane, commonly found in cruciferous plants, can prevent, delay or reverse precancerous lesions by inducing type II detoxification enzymes. Studies have found that sulforaphane can not only inhibit and kill cancer cells, but also has anti-oxidation, immune regulation, anti-inflammation, and prevention of cardiovascular diseases. This makes sulforaphane one of the most concerned natural products in the world. However, sulforaphane is unstable and is easily affected by environmental factors such as temperature, oxygen, light, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A23P10/30
CPCA23V2002/00A23L33/105A23P10/30A23V2250/21A23V2300/10A23V2300/14
Inventor 栾连军黄忆真刘雪松吴永江谈满良
Owner ZHEJIANG UNIV