Detection method for determining pregnancy of sow by using sow urine

A detection method and technology for sows, applied in the field of pig breeding, can solve the problems of low accuracy, loss of early diagnosis of pregnancy, difficult implantation of fertilized eggs, etc., and achieve the effect of convenient operation

Active Publication Date: 2018-12-21
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] This method is to use exogenous hormone injection to prompt the sow to show estrus symptoms, but it must also be combined with the boar's test and appearance to judge whether it is pregnant. Although the time can be advanced to about 21 days, the injection of hormone has no effect on breeding. After the sow is a great stress, it is easy to cause the uterus of the pregnant sow to contract, making it difficult for the fertilized eggs to implant, and the accuracy is not high
[0010] The above 4 detection methods can basically judge whether pregnancy is 21 days after mating in terms of time, and the accuracy is not high; currently the most commonly used method is B-ultrasound detection, although the accuracy has improved, but its detection time is 30 days after mating Left and right, lost the significance of early diagnosis of pregnancy

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  • Detection method for determining pregnancy of sow by using sow urine
  • Detection method for determining pregnancy of sow by using sow urine
  • Detection method for determining pregnancy of sow by using sow urine

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Experimental program
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Effect test

Embodiment 1

[0058] In order to detect whether mated sows of Large White pigs are pregnant or not, urine samples were collected from 7 large white sows on the 10th day after mating, and urine samples from unmated sows and boars were collected as the first negative control. For the sample and the positive control sample, prepare 50ul ultrapure water as the second negative control sample, and number the samples respectively to detect the urine SRY gene. The specific operation steps are as follows:

[0059] (1) Urine collection and centrifugation of mating sows

[0060] Take 7 parts of fresh large white sow urine, 1 part of gilt urine, and 1 part of boar urine collected in 1.5ml centrifuge tubes, put them in a centrifuge after numbering, 12000 rpm Centrifuge for 10 minutes, take 50ul of the upper transparent liquid, put it into a new centrifuge tube for later use, and number it;

[0061] (2) Design a pair of specific primers

[0062] The primers are synthesized according to the sex determin...

Embodiment 2

[0081] In order to detect whether Duroc mated sows are pregnant or not, urine samples were collected from 7 Duroc sows on the 10th day after mating, and at the same time, 1 part of urine from unmated sows and 1 part of boar urine were collected as the first negative For the control sample and the positive control sample, prepare 50ul of ultrapure water as the second negative control sample, and number the samples respectively to detect the SRY gene in urine. The specific operation steps are as follows:

[0082] (1) Urine collection and centrifugation of mating sows

[0083] Take 1ml of the collected 7 samples of fresh sow urine to be tested, 1 sample of unmated sow urine (the first negative control sample), and 1 sample of boar urine (positive control sample) into a 1.5ml centrifuge tube , and numbered, immediately put in the centrifuge, 12000 rpm / centrifuge for 10 minutes, take 50ul of the upper transparent liquid and put it into a new centrifuge tube for later use;

[0084]...

Embodiment 3

[0104] In order to detect whether the mated sows of the local breeds Wei pig and Dingyuan pig are pregnant, the urine samples of 3 sows of Wei pig were collected on the 10th day after mating in Guangde Wei pig breeding farm, and the unmated pigs of Wei pig were collected at the same time 1 part of sow urine was used as the first negative control sample of polder pigs, and 1 part of urine of polder boars was collected as a positive control sample of polder pigs; 4 samples of the 10th day after mating were collected in Dingyuan County Ankang Agriculture and Animal Husbandry Co., Ltd. Dingyuan pig sow urine sample, at the same time collect 1 part of Dingyuan pig urine as a negative control of Dingyuan pig, collect 1 part of Dingyuan pig urine as a positive control sample of Dingyuan pig, In addition, 50 ul of ultrapure water was prepared for the second negative control sample, and the samples were numbered respectively to detect the SRY gene in urine. The specific operation steps ...

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Abstract

The invention relates to a detection method for determining pregnancy of a sow by using sow urine. The detection method for determining the pregnancy of the sow by using the sow urine comprises the following operation steps of centrifuging the urine of the sow which has been bred for 10 days or above; designing one pair of characteristic primers from the 519th bp to the 634th bp of a translation region of a sex-determining region (SRY) gene of the sow; obtaining an in-vitro amplification product of the SRY gene of the sow through polymerase chain reaction; and meanwhile, arranging two negativecontrols and one positive control; carrying out 2% agarose gel electrophoresis detection on the in-vitro amplification product; and judging whether the detected sow is pregnant or not according to whether a 116bp stripe of the SRY gene is available or not. The detection method for determining the pregnancy of the sow by using the sow urine, provided by the invention, has the advantages that the pregnancy diagnosis time can be advanced to the 10th day after the saw is bred, the non-productive days of the sow can be reduced, and the production cost can be saved.

Description

technical field [0001] The invention belongs to the technical field of pig breeding, and in particular relates to a detection method for determining sow pregnancy by using sow urine. Background technique [0002] The farrowing rate of sows is an important factor affecting the production efficiency of sows, so accurate pregnancy diagnosis is an effective means to reduce the number of non-productive days of sows. The estrus cycle of a sow is generally 21 days. If it is not possible to determine whether the sow is pregnant within 21 days, the sow will have to wait for another estrus cycle, that is, 21 days before estrus and mating. Therefore, it is necessary to feed 21 days more and feed 1 head The cost of a sow is about 30 yuan per day, and missing a estrous cycle is equivalent to an increase of 630 yuan in cost. Therefore, it is very important to accurately judge whether sows are pregnant or not for pig production. At present, the commonly used methods for sow pregnancy dia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/686
CPCC12Q1/686C12Q1/6888C12Q2600/158C12Q2565/125
Inventor 李庆岗吴义景苏世广
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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