Detection method for determining pregnancy of sow by using sow urine

Abstract

The invention relates to a detection method for determining pregnancy of a sow by using sow urine. The detection method for determining the pregnancy of the sow by using the sow urine comprises the following operation steps of centrifuging the urine of the sow which has been bred for 10 days or above; designing one pair of characteristic primers from the 519th bp to the 634th bp of a translation region of a sex-determining region (SRY) gene of the sow; obtaining an in-vitro amplification product of the SRY gene of the sow through polymerase chain reaction; and meanwhile, arranging two negativecontrols and one positive control; carrying out 2% agarose gel electrophoresis detection on the in-vitro amplification product; and judging whether the detected sow is pregnant or not according to whether a 116bp stripe of the SRY gene is available or not. The detection method for determining the pregnancy of the sow by using the sow urine, provided by the invention, has the advantages that the pregnancy diagnosis time can be advanced to the 10th day after the saw is bred, the non-productive days of the sow can be reduced, and the production cost can be saved.

Description

technical field [0001] The invention belongs to the technical field of pig breeding, and in particular relates to a detection method for determining sow pregnancy by using sow urine. Background technique [0002] The farrowing rate of sows is an important factor affecting the production efficiency of sows, so accurate pregnancy diagnosis is an effective means to reduce the number of non-productive days of sows. The estrus cycle of a sow is generally 21 days. If it is not possible to determine whether the sow is pregnant within 21 days, the sow will have to wait for another estrus cycle, that is, 21 days before estrus and mating. Therefore, it is necessary to feed 21 days more and feed 1 head The cost of a sow is about 30 yuan per day, and missing a estrous cycle is equivalent to an increase of 630 yuan in cost. Therefore, it is very important to accurately judge whether sows are pregnant or not for pig production. At present, the commonly used methods for sow pregnancy dia...

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Problems solved by technology

[0009] This method is to use exogenous hormone injection to prompt the sow to show estrus symptoms, but it must also be combined with the boar's test and appearance to judge whether it is pregnant. Although the time can be advanced to about 21 days, the injection of hormone has no effect on breeding. After the sow is a great stress, it is easy to cause the uterus of the pregnant sow to contract, making it difficult for the fertilized eggs to implant, and the accuracy is not high

[0010] The above 4 detection methods can basically judge whether pregnancy is 21 days after mating in terms of time, and the accuracy is not high; currently the most commonly used method is B-ultrasound detection, although the accuracy has improved, but its detection time is 30 days after mating Left and right, lost the significance of early diagnosis of pregnancy

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