a for cancer cells and h 2 o 2 Construction of a paper-based sensor for detection
A technology of H2O2, cancer cells, used in instruments, measuring devices, scientific instruments, etc.
Inactive Publication Date: 2020-08-28
UNIV OF JINAN
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But so far, there is little research on the analysis of intracellular H by electrochemiluminescence 2 o 2 research, especially the use of electrochemiluminescence analysis on paper chip devices to realize the detection of cells and their released H 2 o 2 On-site testing has not been reported
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[0027] A method for MCF-7 cancer cells and H 2 o 2 Construction of paper-based sensors for detection:
[0028] (1) Use wax printing technology to print patterns on chromatographic paper. Put the printed wax pattern at a constant temperature of 150 ºC for 2 minutes. The area where the wax is not printed is the hydrophilic area. The shape of the hydrophobic barrier on the three-dimensional origami is determined by Adobe illustrator CS6 software designed, the origami device consists of a common silver / silver chloride reference electrode, carbon counter electrode and carbon working electrode, in addition, it contains an auxiliary region (8 mm in diameter) and a working region (8 mm in diameter), Fold along the fold line (width 1 mm);
[0029](2) Heat 80 mL 0.24 mmol / L chloroauric acid solution to 96 ºC, keep it for 1 min, then add 2.8 mL 34 mmol / L fresh sodium citrate, react for 10 min, the color becomes wine red, Au seed solution preparation If successful, 35 μL of Au seed sol...
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The invention discloses a construction method of a paper-based sensor for detecting cancer cells and H2O2. An electrochemical luminescence (ECL) cell sensor on a microfluidic paper-based analytical device (Mu-PAD) is developed, and the cancer cells and the H2O2 released from the cancer cells are detected sensitively by using an in-situ hydroxyl radical (.OH) DNA cutting method. Good electric conductor Au nano particles are used in Ru@SiO2-Au nanocomposite material so as to enhance electrochemical luminescence signals. In addition, AuPd nanoparticles catalyze the H2O2 released from the inner parts of the cells, generated .OH cuts DNA chains I, and as a result, a large number of ferrocene molecules modified on the DNA I leave the surface of a working area, so that electrochemical luminescence signals are significantly reduced. According to the construction method, it is proved that a good linear relationship between the electrochemical luminescence intensity and the number (1.0x10<2>-5.0x108 cells / mL) of the cancer cells is showed, and meanwhile, a good linear relationship between the electrochemical luminescence intensity and the concentration (200 [rho]mol / L-1[Mu]mol / L) of the H2O2is further showed. The detection limit of a paper-based electrochemical luminescence biosensor is 30 cell / mL, high sensitivity, high specificity, good stability and other good analytical performanceare achieved, and potential application value for cell biology and pathophysiology is achieved.
Description
technical field [0001] The present invention relates to intracellular hydrogen peroxide (H 2 o 2 ) detection method, specifically a microfluidic paper-based analysis device (μ-PAD) based on the characteristics of a built for the determination of the number of cancer cells and the release of H 2 o 2 An electrochemiluminescence sensor of the content. Background technique [0002] h 2 o 2 It is a common and important molecule present in different biological tissues, and it is also involved in various functions and signal transduction processes of cells. intracellular H 2 o 2 Plays an intrinsic role in the regulation of diverse biological processes such as aging and carcinogenesis. Under normal physiological conditions, intracellular H 2 o 2 With a rather low physiological concentration, too high a concentration of H 2 o 2 It can cause various diseases, such as cardiovascular disease, tumor, neurodegeneration and so on. Therefore, it is possible to sensitively and q...
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IPC IPC(8): G01N21/76G01N27/30G01N27/327
CPCG01N21/76G01N27/30G01N27/3278
Inventor 鉴燕楠葛慎光孙晓路王贺赵金歌王绍鹏张彦于京华崔康
Owner UNIV OF JINAN