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Applications of fasudil in drugs for treatment of acute CVB3 viral myocarditis and kits

A technology of viral myocarditis and fasudil, which is applied in the field of medicine to achieve the effect of reducing expression and reducing replication

Inactive Publication Date: 2019-01-01
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the effect of fasudil on acute viral myocarditis after CVB3 infection has been rarely reported at home and abroad

Method used

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  • Applications of fasudil in drugs for treatment of acute CVB3 viral myocarditis and kits
  • Applications of fasudil in drugs for treatment of acute CVB3 viral myocarditis and kits
  • Applications of fasudil in drugs for treatment of acute CVB3 viral myocarditis and kits

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Embodiment Construction

[0025] Hereinafter, the present invention is described in more detail to facilitate understanding of the present invention.

[0026] Experimental animals, cells and virus strains: 90 4-week-old SPF grade male BALB / c mice, weighing (16.3±1.3) grams. Coxsackievirus B3 (CVB3 Nancy strain) was purchased from ATCC, USA. The virus was activated and multiplied by Hep2 cells (Shanghai Institute of Biochemical Cells) with a titer of 10 7 / 0.1ml (TCID 50 ).

[0027] Main drugs and reagents: Fasudil Hydrochloride (trade name: Chuanwei, molecular formula: C 14 h 17 N 3 o 2 S Cl, National Pharmaceutical Accreditation H20040356, batch number 1606241, specifications: 2ml: 30mg) produced by Tianjin Hongri Pharmaceutical Co., Ltd., Trizol total RNA extract and PVDF membrane (Invitrogen, USA), reverse transcription and conventional PCR kit (Thermo Fei Shanghai Company), rabbit anti-mouse IL-17 monoclonal antibody, rabbit anti-mouse ROCK2 antibody (Santa Cruz, USA), IL-17 ELISA kit (Shang...

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Abstract

The invention discloses applications of fasudil in drugs for treatment of acute CVB3 viral myocarditis and kits for inhibiting the expression of inflammatory factors of viral myocarditis. According tothe present invention, the fasudil inhibits Rho associated kinase, and can reduce the expression of inflammatory cytokine IL-17 so as to provide a certain protection effect on CVB3-induced acute viral myocarditis in mice, such that the new idea is developed for the treatment of acute viral myocarditis.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of fasudil in the medicine for treating acute coxsackie B3 virus myocarditis and the application in Rho kinase inhibitor. Background technique [0002] Viral myocarditis (VMC) is mostly caused by Coxsackievirus B3 (CVB3) infection. The massive replication of the virus in cardiomyocytes not only causes direct damage, but also stimulates immune cells to produce a variety of cytokines to mediate immune damage. . It is traditionally believed that the imbalance of Th1 / Th2 cell ratio may be one of the factors affecting the progression and outcome of viral myocarditis. In recent years, it has been found that interleukin-17 (IL-17) produced by type 17 T helper cells (Th17 cells) increases significantly in both acute and chronic viral infection stages of myocarditis, suggesting that during the pathogenesis of viral myocarditis, IL-17 17 is also involved in the inflammatory respon...

Claims

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Application Information

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IPC IPC(8): A61K31/551A61P31/14A61P9/00
CPCA61K31/551A61P9/00A61P31/14
Inventor 李嘉戴克智李西
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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