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A broad absorption spectrum recombinant photosynthetic protein molecule and its construction method

A technology of absorption spectrum and photosynthetic protein, which is applied in the field of wide absorption spectrum recombination of photosynthetic protein molecules and its construction, can solve the problems of narrow absorption spectrum range and achieve broad absorption spectrum range, wide absorption range and wide sources Effect

Active Publication Date: 2020-11-06
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention provides a broad absorption spectrum recombinant photosynthetic protein molecule and its construction method, aiming to solve the problem of narrow absorption spectrum range of biological dye sensitizers in the prior art

Method used

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  • A broad absorption spectrum recombinant photosynthetic protein molecule and its construction method
  • A broad absorption spectrum recombinant photosynthetic protein molecule and its construction method
  • A broad absorption spectrum recombinant photosynthetic protein molecule and its construction method

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Experimental program
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Effect test

Embodiment 1

[0039] PCR amplification of target gene

[0040]Using pUC19-LhcⅡ as template LHCⅡ-F and LHCⅡ-PCA-R as primers, pCDFDuet-cpcA-cpcEF-hox1-pcyA plasmid as template LHCⅡ-PCA-F and PCA-R as primers to amplify LhcⅡ and cpcA genes respectively, The theoretical values ​​of the target bands are 687bp and 492bp respectively. detected by electrophoresis as figure 1 As shown, referring to the Marker as D2000, it is found that the bands are clear under ultraviolet irradiation. The actual size of the LHC II gene is basically consistent with the theoretical value at the position slightly lower than the third band of Marker at 750bp, and the actual size of the PCA gene is in the fourth band of the Marker. The band 500bp is basically consistent with the theoretical value, figure 1 Among them, a is LhcⅡ fragment; b is Marker; c is cpcA fragment.

Embodiment 2

[0042] Overlap PCR

[0043] The LhcII and cpcA genes recovered from the gel were used as templates for overlapping PCR to connect the two fragments. The size of the LHCⅡ-PCA fusion fragment is 1200bp. After electrophoresis detection, the reference marker is D2000. It is found that the size of the target band is slightly above 1000bp, which is basically consistent with the theoretical value of 1200bp, which proves that the fusion fragment is connected successfully. The experimental results are as follows figure 2 as shown, figure 2 Among them, a is the LhcⅡ-cpcA fragment; b is Marker:

Embodiment 3

[0045] double digestion

[0046] Using Marker (1kb) as a reference, the plasmid vectors pCDFDuet-cpcA-cpcEF-hox1-pcyA and pCDFDuet-cpcA-cpcEF-hox1-pebS after BamHI and SacⅠ double digestion, can see clear large molecular weight target band fragments, And it is basically consistent with the theoretical value of 6kb, and there are excised small gene fragments at the lower molecular weight, the results are as follows image 3 as shown, image 3 Among them, a is the carrier containing PEB; b is the excised small gene fragment; c is the carrier containing PCB; d is the excised small gene fragment:

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Abstract

The present invention disclosed a wide absorption spectrum restructuring optical protein molecule, which is combined by reorganized chlorophyll binding protein, algae biliary protein fusion protein and chlorophyll A in vitro.Internal combination catalytic technology, build chlorophyll binding protein, full -color base algae bilobulin fusion molecules, and perform heterogeneous expression in the BL21 of E. coli Engineering strain, and then combine chlorophyll A with chlorophyll combined with protein and algae biliary protein fusion molecules for in vitroReorganization, obtaining a wide -absorbing spectrum allergenic agent that includes both LHCII and algae biliary protein absorption spectrum, provides a mature method for large -scale reorganization of algae biliary protein and LHCI Ⅱ pigment protein molecules, and for exploring the allergic solar cells of biological dyeing solar cellsApplication and broadening dye sensitizer absorption spectral range provides new ideas.

Description

technical field [0001] The invention relates to the field of biological materials, and relates to a photosynthetic protein molecule, in particular to a broad absorption spectrum recombined photosynthetic protein molecule and a construction method thereof. Background technique [0002] Both energy crisis and environmental problems are increasingly urgent and important issues facing mankind. With the decrease of non-renewable resources and the continuous aggravation of environmental pollution, the task of developing new clean energy is imminent. Solar energy is an inexhaustible, environmentally friendly and widely distributed renewable resource. Researchers from various countries have always been very keen on developing and utilizing solar energy and applying it to all aspects of human life. [0003] Dye-sensitized solar cells (Dye Sensitized Solar Cell, DSSC) appeared after silicon series solar cells and compound thin-film solar cells, and are typical third-generation solar c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/70
CPCC12N15/62C12N15/70C07K14/405C07K14/415C07K14/795C07K2319/00C07K14/195
Inventor 葛保胜于倩侯琪琪
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)