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Method for preparing microbial agent for controlling root rot of fruit trees

A microbial inoculum and root rot technology, applied in the field of fertilizers, can solve the problem that root rot cannot be completely cured, and achieve the effects of eliminating heavy metal pollution, long survival time, and reducing planting costs.

Pending Publication Date: 2019-01-04
山西永丰生物工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of this, the object of the present invention is to provide a preparation method of microbial inoculant for preventing and treating fruit tree root rot, aiming at overcoming the defect that current root rot cannot be completely cured

Method used

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  • Method for preparing microbial agent for controlling root rot of fruit trees

Examples

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Embodiment 1

[0016] A preparation method for preventing and treating fruit tree root rot comprising the following steps:

[0017] S1: Selection of strains: Select qualified and excellent Rhodopseudomonas palustris and Rhodobacter sphaericus to form pure strains of photosynthetic bacteria, including 60 parts of Rhodopseudomonas palustris and 10 parts of Rhodobacter sphaericus;

[0018] S2: The configuration and sterilization of primary medium: add 40g of yeast extract, 100g of ammonium sulfate, 100- of glucose, 50g of potassium dihydrogen phosphate, 10g of dipotassium hydrogen phosphate, 1g of magnesium sulfate, and 5g of calcium chloride to 100kg of In water, adjust the pH value of the mixed aqueous solution to 6.8, then sterilize at 100°C for 20 minutes, then cool to 30°C for use;

[0019] S3: primary fermentation culture: under sterile conditions at 30°C, select 500ml of pure photosynthetic bacteria prepared in step S1, inoculate it into the primary culture medium configured in step S2 a...

Embodiment 2

[0024] A preparation method for preventing and treating fruit tree root rot comprising the following steps:

[0025] S1: Selection of strains: select qualified and excellent Rhodopseudomonas palustris and Rhodobacter sphaericus to form pure strains of photosynthetic bacteria, including 80 parts of Rhodopseudomonas palustris and 20 parts of Rhodobacter sphaericus;

[0026] S2: The configuration and sterilization of primary medium: add 50g of yeast extract, 300g of ammonium sulfate, 300g of glucose, 100g of potassium dihydrogen phosphate, 50g of dipotassium hydrogen phosphate, 10g of magnesium sulfate, and 10g of calcium chloride into 100kg of water , adjust the pH value of the mixed aqueous solution to 7, and then sterilize it at a high temperature of 121°C for 30 minutes and then cool it to 35°C for use;

[0027] S3: primary fermentation culture: under sterile conditions at 35°C, select 500ml of pure photosynthetic bacteria prepared in step S1, inoculate it into the primary me...

Embodiment 3

[0032] A preparation method for preventing and treating fruit tree root rot comprising the following steps:

[0033] S1: Selection of strains: select qualified and excellent Rhodopseudomonas palustris and Rhodobacter sphaeroides to form pure strains of photosynthetic bacteria, including 70 copies of Rhodopseudomonas palustris and 30 copies of Rhodobacter sphaeroides;

[0034] S2: The configuration and sterilization of primary medium: add 60g of yeast extract, 300g of ammonium sulfate, 300g of glucose, 150g of potassium dihydrogen phosphate, 80g of dipotassium hydrogen phosphate, 15g of magnesium sulfate, and 20g of calcium chloride to 100kg of water , adjust the pH value of the mixed aqueous solution to 7.2, and then sterilize it at a high temperature of 130°C for 35 minutes and then cool it to 40°C for use;

[0035] S3: primary fermentation culture: under aseptic conditions at 40°C, select 500ml of the pure strain of photosynthetic bacteria prepared in step S, inoculate it into...

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Abstract

The invention relates to a method for preparing a microbial agent for controlling root rot of fruit trees. The method comprises the following steps: selecting culture, preparation and sterilization treatment of a primary culture medium, primary fermentation culture, secondary fermentation culture, third-level fermentation culture and fourth-level fermentation culture, and finally fermenting to form the microbial agent of which the photosynthetic bacteria count is more than or equal to 2 billion per ml. After being applied to soil, the microbial agent fertilizer disclosed by the invention is capable of realizing rapid mass propagation, the photosynthetic bacteria can effectively kill root rot fungi and other harmful bacteria by virtue of the principle of controlling bacteria with bacteria,the pesticide and fertilizer residues are degraded, the heavy metal pollution is eliminated, the soil is improved and remediated, and a good soil eco-environment is provided for root system growth ofthe fruit trees. Therefore, tree health is restored, and the effect of controlling the root rot is achieved.

Description

technical field [0001] The invention belongs to the field of fertilizers, and in particular relates to a preparation method of a microbial inoculum, in particular to a preparation method of a microbial inoculum for preventing and treating fruit tree root rot. Background technique [0002] Due to the long-term extensive use of chemical fertilizers and pesticides, the soil for fruit tree planting has been severely compacted and acidified, resulting in a large number of fruit tree root rot pathogens. Root rot occurs widely, seriously affecting the healthy and sustainable development of the fruit tree planting industry, and causing huge economic losses to fruit farmers. Measures such as soil application of fungicides and digging roots to dry are traditional methods for treating fruit tree root rot for many years, which are not only labor-intensive, but also require a lot of investment and little effect. Therefore, fruit tree root rot plagues fruit farmers like "cancer", serious...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/01
CPCC05F11/08C12N1/20
Inventor 马文虎李保珍郭朝阳
Owner 山西永丰生物工程技术有限公司