Method for separating plasma chylomicrons

A technology of chylomicron and separation method, applied in the field of separation of plasma chylomicron

Active Publication Date: 2019-01-04
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention intends to provide a method for separating chy...

Method used

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  • Method for separating plasma chylomicrons
  • Method for separating plasma chylomicrons
  • Method for separating plasma chylomicrons

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for separating plasma chylomicrons of the present invention comprises the following steps:

[0038] A. Frozen plasma: put the plasma in the plasma bag together with the plasma bag into the freezer of the refrigerator to freeze at -40°C, and take it out after freezing for 8 hours;

[0039] Specifically, blood is drawn to such as figure 1 In the plasma bag shown, as figure 1 As shown, the blood plasma bag includes a bag body 25, and the top of the bag body 25 is communicated with a feeding tube 28, combined with figure 2 It can be seen that the bottom of the bag body 25 is horizontally equipped with a first bottom plate 14, and the right side of the first bottom plate 14 is horizontally equipped with a second bottom plate 19. Rubber mat. The first rotating shaft 1 is mounted on the bag body 25 for sealing and rotating, and the right side of the first rotating shaft 1 is installed for sealing and rotating a second rotating shaft 24. A connecting rod 23 is deta...

Embodiment 2

[0047] The difference between this example and Example 1 is that the plasma freezing time is 8 hours, and the freezing temperature is -40°C.

Embodiment 3

[0049] The difference between this example and Example 1 is that the resting temperature of the plasma is 0° C., and the resting time is 8 hours.

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Abstract

The invention relates to the technical field of medical treatment, in particular to a method for separating plasma chylomicrons. The method comprises the following steps of A, freezing plasma, whereina plasma bag containing plasma is frozen, freezing time is 2-8 hours, and the freezing temperature is 40 DEG C-10 DEG C; B, standing plasma, wherein the frozen plasma bag in step A is placed upside down, and standing is conducted at 0 DEG C-4 DEG C for 8-12 hours; C, centrifuging, wherein after standing in step B, the plasma is centrifuged in a centrifuge, the centrifuge has a centrifugal force of 3900 g-4200 g, the centrifugation time is 8-12 minutes, and the centrifugation temperature is 0 DEG C-4 DEG C; D, separating, wherein the plasma in the plasma bag after centrifugation in step C is extracted to achieve separation of the plasma and the chylomicrons. The method solves the problem of separating the chylomicrons from the plasma.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for separating plasma chylomicrons. Background technique [0002] In the prior art, in the blood donated for free when drawing blood, if the content of chylomicrons (the largest lipoprotein particle in human plasma) in the plasma exceeds the standard, the plasma will be discarded directly, resulting in a large amount of waste of plasma. Separation of chylomicrons to make the content reach the specified standard will save a large amount of plasma. However, methods for isolating chylomicrons from plasma do not exist in the prior art. [0003] At present, there are scholars in Guangzhou and Shandong who have carried out the preparation technology of high-fat blood, but only the technology of removing the high-fat plasma in the suspended red blood cells as a whole, and no research reports have been found on the removal of chylomicrons by high-fat plasma itself , because t...

Claims

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Application Information

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IPC IPC(8): G01N1/38
CPCG01N1/38
Inventor 易中梅徐婷
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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