Kit for measuring concentration of free light chain Lambda and preparation method

A free light chain and kit technology, applied in the biological field, can solve the problems of poor sensitivity and repeatability, achieve strong affinity, improve repeatability and sensitivity

Inactive Publication Date: 2019-01-08
苏州普瑞斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the determination of free light chains, Lambda free light chains are mainly determined by latex-enhanced turbidimetric method in China at present; however, in clinical applications, the

Method used

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  • Kit for measuring concentration of free light chain Lambda and preparation method
  • Kit for measuring concentration of free light chain Lambda and preparation method
  • Kit for measuring concentration of free light chain Lambda and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A test kit for measuring the concentration of free light chain Lambda, including reagent R1 and reagent R2, each component and concentration of the reagent R1 include dihydrate sodium dihydrogen phosphate as the first buffer A, and its concentration is 0.8g / L, as the disodium hydrogen phosphate dodecahydrate of the first buffer solution B, its concentration is 24.31g / L, as the sodium chloride of the first electrolyte, its concentration is 5.8g / L, as the first polymer accelerator PEG 6000, its concentration is 40g / L, and Proclin-950 as the first preservative, its concentration is 2.0%, each component and concentration of described reagent R2 include dihydrogen phosphate dihydrate as the second buffer solution A Sodium at a concentration of 0.5 g / L, disodium hydrogen phosphate dodecahydrate at a concentration of 23.4 g / L as the second buffer B, sodium chloride at a concentration of 5.8 g / L as the second electrolyte, As the bovine serum albumin of the second stabilizer, i...

Embodiment 2

[0045] A test kit for measuring the concentration of free light chain Lambda, including reagent R1 and reagent R2, each component and concentration of the reagent R1 include dihydrate sodium dihydrogen phosphate as the first buffer A, and its concentration is 3.2g / L, as the disodium hydrogen phosphate dodecahydrate of the first buffer solution B, its concentration is 8.75g / L, as the sodium chloride of the first electrolyte, its concentration is 20.0g / L, as the first polymer accelerator PEG 6000, its concentration is 80g / L, as the Proclin-300 of the first preservative, its concentration is 0.8%, each component and concentration of described reagent R2 include as the second buffer solution A sodium dihydrogen phosphate dihydrate , its concentration is 2.8g / L, as the second buffer solution B, disodium hydrogen phosphate dodecahydrate, its concentration is 11.5g / L, as the second electrolyte sodium chloride, its concentration is 20.0g / L, as The casein of the second stabilizer, its...

Embodiment 3

[0056] A test kit for measuring the concentration of free light chain Lambda, including reagent R1 and reagent R2, each component and concentration of the reagent R1 includes sodium dihydrogen phosphate dihydrate as the first buffer A, and its concentration is 2g / L , as the disodium hydrogen phosphate dodecahydrate of the first buffer solution B, its concentration is 16g / L, as the sodium chloride of the first electrolyte, its concentration is 12g / L, as the PEG 6000 of the first polymer accelerator, Its concentration is 120g / L, as the thimerosal of the first preservative, its concentration is 1%, and each component and concentration of described reagent R2 include dihydrate sodium dihydrogen phosphate as the second buffer solution A, and its concentration is 1.5 g / L, disodium hydrogen phosphate dodecahydrate at a concentration of 17 g / L as the second buffer B, sodium chloride at a concentration of 13 g / L as the second electrolyte, gelatin as the second stabilizer, Its concentra...

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Abstract

The invention relates to the technical field of biology, in particular to a kit for measuring concentration of free light chains Lambda and a preparation method. According to the technical scheme, thekit for measuring the concentration of the free light chains Lambda is characterized by containing a reagent R1 and a reagent R2, wherein biotin and streptavidin are added to the reagent R2, biotin binds with free light chain Lambda antibodies in a ratio of biotin to the free light chain Lambda antibodies being 2:1, and streptavidin binds with biotin-goat anti-human free light chain Lambda antibodies in a ratio of streptavidin to the biotin-goat anti-human free light chain Lambda antibodies being 1:1. The kit for measuring the concentration of the free light chains Lambda has the advantages as follows: a cascade amplification reaction is adopted, that is, the goat anti-human free light chain Lambda antibodies are linked with biotin in a ratio of the goat anti-human free light chain Lambdaantibodies to biotin being 1:2 and then subjected to a mixed reaction with streptavidin in a ratio of the biotin-goat anti-human free light chain Lambda antibodies to streptavidin being 1:1, repeatability and sensitivity can be obviously improved, and the linear range can reach 4-140 mg/L.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit and a preparation method for measuring the concentration of free light chain Lambda. Background technique [0002] Immunoglobulin (Ig) light chains are divided into two types, kappa (kappa) and lambda (lambda), each Ig molecule has only one type of light chain, and the ratio of human kappa (kappa) and lambda (lambda) is 6:4. Light chains are small molecular proteins that can freely pass through the glomerular basement membrane, and are reabsorbed back into the blood circulation in the renal tubules, so only a small amount of light chains exist in normal human urine. When metabolic disorders and multiple myeloma occur, a large number of free light chains will appear in the blood and be excreted in the urine, which is Bence-Jones protein. Normal human serum free light chain concentration is 3-19 mg / L, of which λ-type free light chain is 6-26 mg / L, and the κ / λ ratio is 0.26-1.65...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6857G01N2333/47
Inventor 袁嘉扬吴朝晖杜微微李磊
Owner 苏州普瑞斯生物科技有限公司
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