Cotton cytoplasmic-nuclear sterile line motorized sprayer blowing breeding hybridization seed production method
A hybrid seed production and sprayer technology, applied in the field of crop cultivation, can solve the problems of heavy workload, rising labor costs, and declining cotton hybrids, and achieve good economic and social benefits, increase cotton production, and increase income.
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Embodiment 1
[0010] Example 1: Cotton plasmon-nuclear interaction male sterile line bumblebee pollination reproduction, in the breeding field, plant the transgenic insect-resistant plasmon-nuclear interaction male sterile line JA-07 and the corresponding maintainer line JB-07, the male sterile line JA-07 is The female parent, the maintainer line JB-07 is the male parent, each row of female parent corresponds to each row of male parent, planted at intervals, the ratio of parent to parent is 1:1, corn is planted around the breeding field for isolation and no other lines are planted within one kilometer of the breeding field of cotton. At 9:00-10:00 a.m. per mu of breeding field during the current flowering period (the time when the pollen activity is the best), use a motorized sprayer to blow air from the maintainer or restorer line to the sterile cotton plant to suspend the pollen in the air for a short time , the CMS completes pollination. Aug. 25 - Aug. 30 Cessation of pollination with m...
Embodiment 2
[0011] Example 2: Cotton plasmon-nuclear interactive sterile line bumblebee pollination hybrid seed production, in the seed production field, plant the transgenic insect-resistant insect-plasmic nuclear interactive sterile line JA-07 and the corresponding restorer line JR-31, male sterile line JA -07 is the female parent, and the restoration line JR-31 is the male parent. Each row of female parent corresponds to each row of male parent. The ratio of parent to parent is 1:1. Other strains of cotton are grown. During the current flowering period, 9:00-10:00 a.m. (period of the best pollen activity) in the field per mu, use a motorized sprayer to blow air from the restorer line to the sterile line cotton plants, so that the pollen is suspended in the air for a short period of time, making them sterile. Pollination is complete. Aug. 25 - Aug. 30 Cessation of pollination with motorized sprayer blowing (stop pollination). When harvesting flowers, the seeds collected from the steri...
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