Application of diosgenin in the preparation of drugs for inhibiting vascular calcification
A technology of diosgenin and vascular calcification, which is applied in the field of medicine and can solve the problems that diosgenin has not been reported.
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Embodiment 1
[0021] 1 Animal model establishment
[0022] All animal care and experimental procedures are carried out in accordance with the requirements of the Animal Management and Use Committee (IACUC) of the Laboratory Animal Science Center of Dalian Medical University. Select 8-week-old SPF female LDLr - / - Mice conduct research. The environment of the animal room is set at a temperature of (22-25°C), and the white night cycle is 12 hours / 12 hours a day. The animals can freely draw water and food in the cage. The mice have a week to adapt to the new environment, and then continue as follows 12 weeks.
[0023] 2 Construction and intervention of vascular calcification model
[0024] The mice were randomly divided into five groups: the first group (n=6) only received normal diet and ordinary feed (blank group); the second group (n=6) normal diet and ordinary feed, diosgenin 35mg / kg daily gavage One time (Dioscin; Nanjing Jingzhu Biotechnology Co., Ltd.) (Dioscin is dissolved in 0.5% carboxyme...
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