Bacillus siamensis and application of the same in controlling tomato root-knot nematode disease

A technology of tomato root-knot nematode and Bacillus, applied in the application, nematicide, bacteria and other directions, can solve the problems of high multiple cropping index, slow action, complex soil environment, etc., to achieve strong stress resistance, robust growth, and novel strains Effect

Inactive Publication Date: 2019-01-11
青岛秾贝尔生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect is slow, the control effect is not good, and it cannot meet the market demand
Due to the high replanting index of facility vegetables, small nematodes, tenacious vitality,

Method used

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  • Bacillus siamensis and application of the same in controlling tomato root-knot nematode disease
  • Bacillus siamensis and application of the same in controlling tomato root-knot nematode disease
  • Bacillus siamensis and application of the same in controlling tomato root-knot nematode disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Mutation screening and identification of Bacillus siamese strain LK0006

[0023] 1. Mutagenesis and screening of strains

[0024] (1) Isolation of starting strain LK0000

[0025] Isolation of Bacillus: Soil sample collection, in the tomato planting greenhouse plot of Baojia Village, Xinjia Street, Longkou City, Shandong Province, using the 5-point sampling method to collect the appropriate amount of soil around and in the center of the plot within a depth of 10-20cm. Mix in equal amounts. Indicate the location, time and person of the collection. Weigh 1g of soil sample into 100mL of sterile water, place it in a shaker at 30°C at 150rpm for 10min, then place it in a water bath at 60°C for 30min, take 100μL of 10 -2 、10 -3 、10 -4 The diluted solution is spread on the LB medium plate, and each gradient is coated in three parallels. After culturing at 30°C for 2 days, the microbial colonies of different forms on the LB medium are picked and streaked on th...

Embodiment 2

[0047] Embodiment 2: preparation fermented liquid

[0048] Among them, the formula of the seed liquid medium: in terms of mass percentage, bean cake powder 3.00%, corn flour 4.00%, glucose 0.40%, fish meal 0.50%, potassium dihydrogen phosphate 0.03%, light calcium carbonate 1.20%, ammonium sulfate 0.30%, chloride Sodium chloride 0.10%, the rest is water, and the pH is adjusted to 7.0-7.2.

[0049] The mass fermentation medium is the same as the seed liquid medium.

[0050] (1) Prepare the seed liquid of Bacillus siamese LK0006: pick a small amount of bacteria from the inclined plane of the test tube of Bacillus siamese LK0006, transfer to the seed liquid culture medium, shake and culture at 28° C. for 24 hours, and this is the seed liquid;

[0051] (2) Inoculate the seed solution prepared in step (1) into a fermenter containing a large amount of sterilized fermentation medium in a sterile environment, cultivate at a constant temperature of 28-30° C. for 36 hours, and the tran...

Embodiment 3

[0052] Example 3: Verification of indoor effect of Bacillus siamese LK0006 fermentation broth on tomato root-knot nematode

[0053] 1. Experimental method

[0054] 1.1 Preparation of root-knot nematode second-instar larvae suspension

[0055] Fresh egg masses of root-knot nematodes were picked from the tomato roots, incubated in a Beman funnel, and collected once after 24 hours for a total of 7 days. The nematode solution was diluted to contain 100 second-instar larvae / mL, and stored at 4°C for later use.

[0056] 1.2 Preparation of egg suspension

[0057] Cut the tomato roots with root-knot nematodes into pieces and put them into a conical flask, add 100mL of 0.5% NaClO solution, shake vigorously for 1-2 minutes, quickly pour them into a 200-500-mesh sieve group and wash them with tap water for 10 minutes, and collect them through a 500-mesh sieve Eggs in the net were diluted to an egg suspension with a concentration of 3000 grains / mL.

[0058] 1.3 Indoor and in vitro mea...

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Abstract

The invention discloses a Siamese bacillus and an application of the same in controlling tomato root-knot nematode disease. The strain is identified as Bacillus siamensis LK0006 (CGMCC No. 15215). Theresults showed that the inhibition rate of nematodes is 90.35% when the fermentation broth is diluted 25 times and treated for 48 hours in vitro. The hatching of eggs could be inhibited by 25, 50, 100 times dilution of fermentation broth. Bacillus siamensis LK0006 wettable powder had a significant control effect on tomato root-knot nematode disease (75.02%) under pot culture condition, and tomatohad strong growth and no pesticide harm, so it had a good prospect in biocontrol of tomato root-knot nematode disease.

Description

technical field [0001] The invention belongs to the field of microorganism application. Specifically, the present invention relates to a strain of Bacillus siamica and its application in preventing and treating root-knot nematode disease of tomato, and a biological control agent prepared by using the Bacillus siamica. Background technique [0002] Tomato (scientific name: Lycopersicon esculentum Mill.) is an annual or perennial herbaceous plant belonging to the family Solanaceae. It is widely cultivated in various parts of China, and many areas have greenhouses or plastic greenhouses for cultivation. In recent years, due to continuous cropping for many years in protected land cultivation, the multiple cropping index has increased, and the heavy cropping has caused the occurrence of tomato root-knot nematodes to become more and more serious. Generally, it can cause a 10%-20% reduction in yield, and it can reach 30%-40% in severe cases, or even Extinction has become one of th...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P5/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 牛兆颖王凤娇张淑凤刘新李宁邱振豪张淑静牛赡光
Owner 青岛秾贝尔生物工程有限公司
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