Molecular breeding method for improving stigma exsertion rate of sterile line of three-line rice
A technology of three-line sterile lines and exposed stigmas, which is applied in the fields of botany equipment and methods, biochemical equipment and methods, and microbial measurement/inspection, etc., and can solve the problems of low yield of hybrid seed production, regulatory analysis, and incomplete research results In order to improve the efficiency and accuracy of improved breeding and speed up the breeding process
Active Publication Date: 2019-01-15
SHANGHAI AGROBIOLOGICAL GENE CENT
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Abstract
The invention discloses a molecular breeding method for improving the stigma exsertion rate of a sterile line of three-line rice. The method comprises the following steps: with the low-stigma-exsertion-rate maintainer line of three-line rice as a recurrent parent and the maintainer line, carrying the stigma exsertion rate gene qES7, of three-line rice as a donor, performing hybridization and backcrossing; performing selection assisted by molecular markers closely linked with the stigma exsertion rate gene qES to obtain individual homozygous genotype plants with significantly improved stigma exsertion rate; and subjecting the individual homozygous genotype plants to hybridization with a wild-abortive cytoplasmic male-sterile line for 3 generations continuously so as to breed the improved sterile line with an increased stigma exsertion rate and paired maintainer lines. With the method, QTL for controlling the stigma exsertion rate can be quickly introduced, selection efficiency is improved, and help is provided for improving the stigma exsertion rate of the sterile line of hybrid rice and the yield of seed production.
Application Domain
Microbiological testing/measurementPlant genotype modification
Technology Topic
Molecular breedingHomozygous genotype +5
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