Method for extracting, separating and purifying polysaccharides from myrtle fruit
A technology for separation and purification of myrtle, applied in the field of extracting and separating and purifying polysaccharides from myrtle fruit, can solve the problems of low development and utilization rate, low level of processing and utilization, and achieve low cost, physical and chemical stability and mechanical properties. More, high yield effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0042] A method for extracting and isolating and purifying polysaccharides from myrtle fruit, comprising the following steps:
[0043] (1) Extract polysaccharides from myrtle fruit: Weigh 110g of dried myrtle fruit and crush them, pass through a 40-mesh sieve, weigh 100g of dry powder, add 1000mL of distilled water, boil in a reflux device for 4h, and take the filter residue after suction filtration Then add 1000 mL of distilled water, boil for 4 h in a reflux device, combine the supernatants, and concentrate under reduced pressure. Slowly add 4 times the volume of 95% (V / V) ethanol to the concentrated solution, and keep stirring with a glass rod to uniformly precipitate the polysaccharide, then put it in a refrigerator at 4°C for 12 hours, and then centrifuge at 5000r / min for 15 minutes to collect the precipitate. After drying, myrtle fruit crude polysaccharide is obtained;
[0044] (2)H 2 o 2 Decolorization method: Weigh 20g myrtle fruit crude polysaccharide, add 100mL di...
Embodiment 2
[0054] The myrtle fruit polysaccharides obtained in Example 1 were subjected to ultraviolet spectrum analysis. Each 1 mg polysaccharide sample was weighed to prepare a 1 mg / mL polysaccharide solution, and the ultraviolet spectrum was scanned in the range of 200-500 nm.
[0055] figure 2 is the UV spectrum of P1, image 3 is the UV spectrum of P2, Figure 4 is the UV spectrum of P3, Figure 5 It is the ultraviolet spectrogram of P4, and the results show that there are no obvious absorption peaks at 260nm and 280nm in the four purified components, indicating that they do not contain protein and nucleic acid substances.
Embodiment 3
[0057] Carry out polysaccharide molecular weight analysis to the myrtle fruit polysaccharide that embodiment 1 obtains, concrete experimental method is as follows:
[0058] Molecular weights were determined using gel permeation chromatography (GPC). Take 2 mg of polysaccharide sample, add 0.02 mol / L phosphate buffer solution to dissolve, prepare 2.0 mg / mL solution, filter with 0.22 μm sterile filter membrane, and take the filtrated liquid for later use. Chromatographic conditions: column temperature 35°C, 0.02mol / L phosphate buffer as mobile phase, flow rate 0.6mL / min, injection volume 20μL, Waters 2410 differential refractive index detector for detection. Prepare a series of dextran solutions with different molecular weights (700, 400, 200, 100, 50, 30, 10, 5kD) as standards, draw a standard curve, and calculate the molecular weight of the sample according to its corresponding elution volume against the standard curve .
[0059] The measured molecular weight of the myrtle f...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More