A Haemophilus parasuis culture medium and a high-density fermentation culture method
A high-density fermentation technology for Haemophilus parasuis, which is applied in the field of Haemophilus parasuis medium and high-density fermentation culture, can solve the problems of affecting the fermentation effect, restricting the high-density fermentation of Haemophilus parasuis, and reducing the content of bovine serum , to achieve the effect of high-density fermentation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0039] Embodiment 1: the influence of different mediums on the fermentation of Haemophilus parasuis
[0040] Adopt four kinds of substratum to carry out Haemophilus parasuis fermentation among the present invention, are respectively:
[0041] Medium I: TSB medium according to weight / volume, (glucose 2.5g / L, peptone 17.0g / L, soytone 3.0g / L, K 2 HPO 4 2.5g / L, NaCl 5.0g / L), NAD 50mg / L, according to the volume ratio, 3.0% bovine serum, and the balance is water;
[0042] Medium II: by weight / volume, glucose 2.5g / L, yeast powder 3.0g / L, tryptone 22.0g / L, K 2 HPO 4 3.0g / L, NaCl 5.0g / L, NAD 50mg / L, according to the volume ratio, 3.0% bovine serum, the balance is water;
[0043] Medium III: by weight / volume, glucose 2.5g / L, peptone 10.0g / L, yeast powder 10.0g / L, K 2 HPO 4 2.5g / L, NaCl 5.0g / L, NAD 50mg / L, according to the volume ratio, 3.0% bovine serum, the balance is water;
[0044] Medium IV: by weight / volume, glucose 2.5g / L, peptone 10.0g / L, yeast powder 10.0g / L, K 2 HPO ...
Embodiment 2
[0049] Example 2: Effects of different serum additions on the fermentation of Haemophilus parasuis and the relationship between capacitance value and bacterial concentration / serum concentration
[0050] 2.1 On-line determination of viable cell concentration and the relationship between the concentration of viable cells and the number of viable bacteria
[0051] Example 1 Medium IV was used as the fermentation medium for Haemophilus parasuis, and Haemophilus parasuis was cultured on a 10L fermenter, and the specific culture process was the same as in Example 1. At the initial stage of culture, the capacitance value of living cells, the concentration of bacteria and the number of viable bacteria were measured at different culture times, and the relationship between the concentration of bacteria and the capacitance value and the number of viable bacteria was calculated. The results are shown in the attached figure 2 shown.
[0052] attached by figure 2 (a) It can be seen that...
Embodiment 3
[0068] Example 3: Application of Serum Supplementation Strategy in Fermentation of Haemophilus parasuis
[0069] Fermentation medium: adopt medium IV in Example 1;
[0070] Haemophilus parasuis type 5 was incubated at 37°C for 18 hours until a single colony grew out.
[0071] Pick a single colony and inoculate it into a 500mL Erlenmeyer flask containing 100mL of seed medium, and incubate for 12 hours at a temperature of 37°C and a stirring speed of 200rpm. According to the inoculation amount of 10%, inoculate in the 10L fermentor that is equipped with 6.0L fermentation medium. The culture temperature was 37°C; the dissolved oxygen level was maintained at 20% by adjusting the stirring speed and ventilation rate; the pH was maintained at 7.5 by using 4mol / L NaOH solution; during the culture process, glucose solution was added to maintain the glucose solution at 1.0g / L, And based on the relationship between the capacitance value change and serum consumption, the acceleration ra...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More