Preparation method and application of a two-step fermentation product with skin repairing effect
A two-step fermentation and skin repair technology, applied in skin care preparations, medical preparations containing active ingredients, pharmaceutical formulations, etc., can solve the problems of many side effects, hinder drug absorption, and high risks, and achieve low cost of raw materials and products. The effect of high added value and broad market prospects
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Embodiment 1
[0046] 1. Solid fermentation of fungi:
[0047] ① Preparation of medicinal culture substrate
[0048] Formula: 64% barley, 7% camellia, 7% camellia, 8% camellia seed, 5% schisandra, 3% bamboo, 2% ginseng, 1% licorice, 1.5% codonopsis, 1.5% medlar.
[0049] Preparation method: mix and pulverize each component according to the formula ratio, add water until the water content is 55% of the total weight; sterilize at 121°C for 1 hour to obtain the medicinal property culture substrate;
[0050] ② Inoculation and fermentation
[0051] Inoculate the liquid strain of the fungus plant Yunzhi CICC50001 (purchased) into 1. the prepared medicinal culture substrate by 5% volume to mass ratio, that is, the added volume of the fungus Yunzhi CICC50001 liquid spawn ( mL) is 5% (mL / g) of the mass (g) of the medicinal culture medium, cultured at 28°C for 25 days to prepare the medicinal fungus;
[0052] 2. Liquid submerged fermentation of Saccharomyces cerevisiae:
[0053] ① Preparation of f...
Embodiment 2
[0060] 1. Solid fermentation of fungi:
[0061] ① Preparation of medicinal culture substrate
[0062] Formula: 72% barley, 5% camellia, 5% camellia, 5% camellia seed, 4% schisandra, 3% bamboo, 1% ginseng, 1.5% licorice, 1.5% codonopsis, 2% wolfberry.
[0063] Preparation method: mix and pulverize each component according to the formula ratio, add water until the moisture content is 51% of the total weight; sterilize at 121°C for 1 hour to obtain the medicinal property culture substrate;
[0064] ② Inoculation and fermentation
[0065] Inoculate the liquid strain of the fungus Yunzhi Bacillus CICC50001 into the medicinal culture substrate prepared by ① according to the volume and mass ratio of 10%, that is, the added volume (mL) of the fungus Yunzhi Bacillus CICC50001 liquid strain is the medicinal property 10% (mL / g) of the mass (g) of the culture substrate, cultivated at 28°C for 28 days to prepare medicinal fungi;
[0066] 2. Liquid submerged fermentation of Saccharomyces...
Embodiment 3
[0074] 1. Solid fermentation of fungi:
[0075] ① Preparation of medicinal culture substrate
[0076] Formula: 68% barley, 5.5% camellia, 5% camellia, 9.5% camellia seed, 3% schisandra, 3.5% bamboo, 2% ginseng, 1.5% licorice, 1% codonopsis, 1% wolfberry.
[0077] Preparation method: mix and pulverize each component according to the formula ratio, add water until the moisture content is 50% of the total weight; sterilize at 121°C for 1 hour to obtain the medicinal property culture substrate;
[0078] ② Inoculation and fermentation
[0079] Inoculate the liquid strain of the fungus Yunzhi Bacillus CICC50001 into the medicinal culture substrate prepared by ① according to the volume and mass ratio of 5%, that is, the added volume (mL) of the fungus Yunzhi Bacillus CICC50001 liquid strain is the medicinal property 5% (mL / g) of the mass (g) of the culture medium, cultivated at 28°C for 25 days to prepare medicinal fungi;
[0080] 2. Liquid submerged fermentation of Saccharomyces ...
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