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Preparation method and application of polyethyleneimine hyperbranched agarose-based boron affinity material

A polyethyleneimine and agarose-based technology, applied in the field of separation, can solve problems such as imperfect identification and separation applications, and achieve high column capacity and operability

Active Publication Date: 2019-01-18
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The method of amplifying boron affinity groups through hyperbranched groups is an effective method to increase affinity and capacity, but existing researches are mostly applied in small molecule and capillary chromatography, and its application in identification and separation at the cellular level is still limited. Imperfect, given that boron affinity materials are necessary for affinity, it is necessary to develop adaptive boron affinity materials for high affinity and high column capacity

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  • Preparation method and application of polyethyleneimine hyperbranched agarose-based boron affinity material
  • Preparation method and application of polyethyleneimine hyperbranched agarose-based boron affinity material
  • Preparation method and application of polyethyleneimine hyperbranched agarose-based boron affinity material

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preparation example Construction

[0042] A preparation method of polyethylenimine hyperbranched agarose-based boron affinity material, comprising the following steps:

[0043] 1) Preparation of agarose microspheres: based on 0.5g of agarose dry powder, dissolve 0.5g of agarose dry powder in 10-20mL of water, stir and boil to a transparent gel, mix well as the water phase; add 3-5g of Span80 Add to 100mL liquid paraffin, mix at a constant temperature of 75-85°C and use it as the oil phase; add the water phase to the oil phase dropwise at a stirring rate of 800-900rpm, then continue stirring at 200-300rpm for 8-12min and then cool to room temperature. Stirring was continued for another 2-8 minutes to form the microspheres. The prepared microspheres were washed with a mixed washing solution of petroleum ether and isopropanol (the volume ratio of petroleum ether and isopropanol is 8-12:1), and then washed with petroleum ether and water respectively to obtain agarose microspheres.

[0044] 2) Preparation of epoxid...

Embodiment 1

[0048] 1. Preparation and characterization of agarose-based and silica-based F-containing boron affinity materials

[0049] 1.1. Preparation of silica gel-based boron affinity material: TMOS (720 μL), GPTMS (520 μL), cetyltrimethylammonium bromide CTAB (216 mg) and 2 mL Acetic acid solution (0.01 M) was slightly shaken for 1 hour to form a uniform solution, which was fully hydrolyzed in an acidic environment.

[0050] The prepared mixed solution containing epoxy groups was respectively reacted with amine-containing compounds to complete the amination, the obtained mixed solution was thoroughly mixed with PEI600 (200 μL), ultrasonicated for 15 min to obtain a homogeneous solution, and then poured into the EP tube, Seal and react in a constant temperature box at 40°C and 70°C for 12 hours respectively.

[0051] After the reaction was completed, the unreacted porogen and other unreacted substances were washed away with 70% methanol aqueous solution. The reacted material was flu...

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Abstract

The invention relates to the technical field of separation, and discloses a preparation method and application of a polyethyleneimine hyperbranched agarose-based boron affinity material. The preparation method comprises the following steps of: step (1) preparing agarose microspheres; step (2) preparing epoxidized agarose; step (3) carrying out amination modification on the epoxidized agarose; andstep (4) preparing phenylboronic acid agarose. According to the prepared agarose-based boron affinity material with high column capacity and high biocompatibility, agarose is selected as a base frame,polyethyleneimine serves as a hyperbranched aglycone for modification, and meanwhile a 3,5-difluoride-4- formylphenylboronic acid is used as an affinity group; and the agarose has relatively high affinity and column capacity, and operability. The preparation method overcomes a series of technical problems and successfully applies a boron affinity material to the identification and separation at the cellular level, and achieves significant progress compared with the prior art.

Description

technical field [0001] The invention relates to the field of separation technology, in particular to a preparation method and application of polyethyleneimine hyperbranched agarose-based boron affinity material. Background technique [0002] The incidence of diseases caused by bacteria is on the rise worldwide and has become an invisible burden in countries all over the world. Therefore, the detection and analysis of bacteria, especially pathogenic bacteria, is of great significance, which requires bacteria with relatively high purity; but the growth environment of bacteria is more complicated, especially the samples are rich in protein, fat, sugar, inorganic salts Such complex matrices pose a huge challenge to bacterial analysis; in addition, the sample matrices are complex and diverse and have no rules to follow, which further increases the difficulty of bacterial purification or detection. For example, based on polymerase chain reaction (polymerase chain reaction, PCR), ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/285B01D15/38B01J20/30
CPCB01D15/3804B01J20/285
Inventor 曹立民郑洪伟林洪隋建新王博成殷佳珞
Owner OCEAN UNIV OF CHINA