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A kind of preparation method and application of ampicillin sensor

A technology of ampicillin and sensors, which is applied in the field of new nano-functional materials and biosensing analysis, can solve the problems of reduced sensitivity, reduced stability and reproducibility of electrochemical sensors, limiting the application of molecularly imprinted membranes, etc., and achieves excellent electron transfer ability, convenient operation, and the effect of reducing testing costs

Active Publication Date: 2021-01-29
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the preparation process of traditional MIP-ECS, there are disadvantages such as difficult elution of template molecules, difficulty in controlling the thickness of imprinted membranes, and poor reproducibility, which limit the application of molecularly imprinted membranes in electrochemical sensors.
These problems, especially the difficult control of the thickness of the molecularly imprinted membrane, which leads to the decrease of the sensitivity of the electrochemical sensor, and the technical difficulties that the molecularly imprinted membrane is easily detached from the electrode surface during the elution process, resulting in a decrease in stability and reproducibility, limit the application of MIP_ECS. It is of great research significance and market value to find new synthesis methods for molecularly imprinted polymers, new methods for modifying molecularly imprinted membrane electrodes, and methods for combining molecularly imprinted membranes with substrate materials to solve the problems of preparation and application of MIP-ECS.

Method used

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  • A kind of preparation method and application of ampicillin sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation of NiFe LDH-nanoarray

[0030] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0031] (2) Weigh 1 mmol Ni(NO 3 ) 2 and Fe(NO 3 ) 3 mixture and 3 mmol urea CO(NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water and stir until clear, then transfer to a 50 mL polytetrafluoroethylene reactor;

[0032] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reactor in step (2), and react at a temperature of 100 °C for 12 hours to prepare nickel-iron bimetallic layered hydrogen Oxide nanosheet array precursor electrode;

[0033] (4) Insert the nickel-iron bimetal layered hydroxide nanosheet array precursor electrode obtained in step (3) into the phosphate buffer solution PBS containing dopamine, ammonium persulfate and cobalt nitrate, and react at a temperatur...

Embodiment 2

[0035] Example 2 Preparation of NiFe LDH-nanoarray

[0036] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0037] (2) Weigh 2 mmol Ni(NO 3 ) 2 and Fe(NO 3 ) 3 mixture and 6 mmol urea CO(NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water and stir until clear, then transfer to a 50 mL polytetrafluoroethylene reactor;

[0038] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reaction kettle in step (2), and react at a temperature of 110 °C for 11 hours to prepare nickel-iron bimetallic layered hydrogen Oxide nanosheet array precursor electrode;

[0039] (4) Insert the nickel-iron bimetallic layered hydroxide nanosheet array precursor electrode obtained in step (3) into the phosphate buffer solution PBS containing dopamine, ammonium persulfate and cobalt nitrate, and react at a...

Embodiment 3

[0041] Example 3 Preparation of NiFe LDH-nanoarray

[0042] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0043] (2) Weigh 3 mmol Ni(NO 3 ) 2 and Fe(NO 3 ) 3 mixture and 9 mmol urea CO(NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water and stir until clear, then transfer to a 50 mL polytetrafluoroethylene reactor;

[0044] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reaction kettle in step (2), and react at a temperature of 130 °C for 9 hours to prepare nickel-iron bimetallic layered hydrogen Oxide nanosheet array precursor electrode;

[0045] (4) Insert the nickel-iron bimetallic layered hydroxide nanosheet array precursor electrode obtained in step (3) into the phosphate buffer solution PBS containing dopamine, ammonium persulfate and cobalt nitrate, and react at a ...

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Abstract

The invention discloses a fabrication method of an ampicillin sensor, and belongs to the technical field of novel nanometer functional material and biological sensing analysis. A nickel-iron thermometal layered hydroxide nanosheet array is fabricated on a disposable throwable electrode, an electronic medium-containing polydopamine thin film and a molecular imprinting polymer taking ampicillin as atemplate molecule polymer are directly and sequentially fabricated on the nickel-iron thermometal layered hydroxide nanosheet array by an in-situ growth method according to large specific surface area, high-activity hydroxyl functional group and amino functional group of polydopamine. After the template molecule is eluted, the original position of the template molecule is changed to holes, and the molecule imprinting polymer of the template molecule is eluted. Therefore, the ampicillin sensor is fabricated and completed.

Description

technical field [0001] The invention relates to a preparation method and application of an electrochemical analysis sensor. It belongs to the field of new nano functional material and biosensing analysis technology. Background technique [0002] Ampicillin, also known as ampicillin, is a β-lactam antibiotic and a semi-synthetic broad-spectrum penicillin that can treat a variety of bacterial infections. Indications include respiratory tract infection, urinary tract infection, meningitis, salmonella infection, and endocarditis. Because of its convenient use and low cost, it is mostly used to treat infectious diseases caused by sensitive bacteria in chickens, such as Escherichia coli, Salmonella, Pasteurella, Staphylococcus and Streptococcus infections. On October 27, 2017, in the list of carcinogens released by the International Agency for Research on Cancer of the World Health Organization, ampicillin was included in the list of category 3 carcinogens. Therefore, the devel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 张勇刘召壹魏琴
Owner UNIV OF JINAN
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