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Method for preventing lesser bougainvillea flower tissue culture seedlings from yellowing

A technology of flower groups and leaves, applied in the field of preventing yellowing of bougainvillea tissue cultured seedlings, can solve problems such as yellowing of bougainvillea tissue cultured seedlings, achieve good sterilization effect, prevent yellowing, and prevent yellowing

Active Publication Date: 2019-01-25
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been reports on the tissue culture of Bougainvillea, but there has been no report on the yellowing of Bougainvillea tissue cultured seedlings.

Method used

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  • Method for preventing lesser bougainvillea flower tissue culture seedlings from yellowing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Method for assaying bougainvillea pollen vigor of the present invention, comprises the steps:

[0022] 1. Selection of explants: At 10 a.m. in the morning on a sunny day, collect the young shoots of the current year on a strong, disease-free mother plant.

[0023] 2. Disinfection treatment: ①Take the collected young shoots back to the laboratory, gently wipe off the epidermal hairs with a rag, and rinse them with tap water; ②Cut them into 6-7cm long sections, put them in a beaker, and cover the beaker with clean gauze ③ Put the soaked and rinsed young shoots into the ultra-clean workbench, first sterilize with 75% alcohol for 30 seconds, and keep shaking the bottle to make the explants fully contact with the alcohol and aseptic Rinse with water 4-5 times; ④ Then sterilize with 0.1% mercuric chloride for 8 minutes, shake the bottle constantly during this period to ensure that each explant can be fully sterilized, and rinse with sterile water 5-6 times.

[0024] 3. Inocu...

Embodiment 2

[0028] 1. Selection of explants: At 10 a.m. in the morning on a sunny day, collect the young shoots of the current year on a strong, disease-free mother plant.

[0029] 2. Disinfection treatment: ①Take the collected young shoots back to the laboratory, gently wipe off the epidermal hairs with a rag, and rinse them with tap water; ②Cut them into 6-7cm long sections, soak them in potassium permanganate for 5 minutes, and soak them in carbendazim 8 minutes, then put it in a beaker, cover the mouth of the beaker with clean gauze, and rinse it under tap water for 2-3 hours; ③ Bring the soaked and rinsed young shoots into the ultra-clean workbench, first disinfect with 75% alcohol for 30 seconds, and keep Shake the bottle to fully contact the explants with alcohol, rinse them with sterile water 4-5 times; ④ then sterilize with 0.1% mercuric chloride + 0.05% Tween 80 for 8 minutes, and keep shaking the bottle during this period to ensure that each explant can be Fully disinfect and r...

Embodiment 3

[0034] 1. Selection of explants: In rainy weather, collect the young shoots of the year on the mother plant that is strong and free of diseases and insect pests.

[0035]2. Disinfection treatment: ①Take the collected young shoots back to the laboratory, gently wipe off the epidermal hairs with a rag, and rinse them with tap water; ②Cut them into 6-7cm long sections, put them in a beaker, and cover them with clean gauze Rinse the mouth of the beaker under tap water for 2-3 hours; ③ Bring the soaked and rinsed young shoots into the ultra-clean workbench, first disinfect with 75% alcohol for 30 seconds, and keep shaking the bottle to make the explants fully contact with the alcohol, without Rinse with bacterial water 4-5 times; ④ Then sterilize with 0.1% mercuric chloride + Tween for 8 minutes, shake the bottle constantly during this period to ensure that each section of explant can be fully sterilized, and rinse with sterile water 5-6 times.

[0036] 3. Inoculation: ① Place the ...

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Abstract

The invention discloses a method for preventing lesser bougainvillea flower tissue culture seedling from yellowing. The method comprises the following steps: (1) choosing an explant; (2) performing sterilizing treatment; (3) inoculating; (4) performing primary culture; (5) performing multiplication culture. The induction rate of the method disclosed by the invention can reach 95% or more, and a multiplication coefficient is 3 to 5; most importantly, the method can prevent lesser bougainvillea flower tissue culture seedlings from yellowing; thus, the tissue culture seedlings can normally differentiate and grow, and large-scale production of the lesser bougainvillea flower can be ensured.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for preventing yellowing of bougainvillea tissue culture seedlings. Background technique [0002] Bougainvillea spectabilia Willa (Bougainvillea spectabilia Willa) is an evergreen climbing or dispersing shrub of the family Mirabilis. It is native to Brazil and is also known as Bougainvillea, Rhododendron, and Baojinhua. There are as many as 300 kinds of cultivars, varieties, and hybrids. Bougainvillea has strong adaptability, various flower colors and long flowering period. It is an important landscaping tree species in the tropics and south subtropics. Bougainvillea is mainly propagated by asexual reproduction, among which cutting and grafting are the most commonly used methods, but some varieties with broad market prospects and high ornamental value have weak growth, weak branch germination, and the survival rate of cuttings and grafting is not high. Its...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008Y02P60/40
Inventor 孙利娜唐庆李冰林茂李进华杜铃杨舒婷陈尔孙开道黄欣廖美兰
Owner GUANGXI FORESTRY RES INST
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