A kind of method of cloud ginseng enzyme processing
A technology of ginseng enzyme and cloud ginseng, which is applied in the field of cloud ginseng enzyme processing, can solve the problems of low added value, inability to give full play to medicinal value, and low economic benefits, and achieve the improvement of protease activity and antioxidant activity, significant health care function, The effect of simple processing technology
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Embodiment 1
[0019] Embodiment 1 preparation of wild ginseng fermentation base liquid
[0020] 1. Determination of enzymatic hydrolysis conditions for single enzymes (pectinase, cellulase, tannase)
[0021] (1) After drying the fresh ginseng, grind it into a fine powder (moisture content ≤ 9.0%).
[0022] (2) Optimum material-to-liquid ratio: Weigh 5 portions of dried ginseng powder of 5g each, put them in a beaker, and add them respectively according to the material-to-liquid ratio of 1:7, 1:8, 1:9, 1:10, and 1:11 After dissolving in ultrapure water, add pectinase (cellulase or tanninase) at 0.5% (mass ratio), enzymolyze at 50°C and pH 5.0 for 180min, measure the enzymolysis rate after the enzymolysis reaction is completed, Determine the optimum material-liquid ratio of pectinase, cellulase and tannase respectively.
[0023] (3) Optimum amount of enzyme added: Weigh 5 parts of ginseng dry powder of 5g each and put them in a beaker, add ultrapure water to dissolve according to the optimu...
Embodiment 2
[0037] Embodiment 2 Lactobacillus plantarum ST fermentation cloud ginseng enzyme process optimization
[0038] 1. Optimum inoculum size: Cool the fermentation base liquid to 30°C-40°C, add sucrose at 9% (mass volume ratio), randomly divide it into 6 experimental groups with equal volume, and then divide them into 1×10 4 cfu / mL, 2.5×10 4 cfu / mL, 5×10 4 cfu / mL, 7.5×10 4cfu / mL, 10 5 cfu / mL, 1.25×10 5 Plantarum ST was inoculated at a concentration of cfu / mL, and fermented at 37°C for 20 hours. After the fermentation, the ginseng enzyme was evaluated sensory to determine the optimal inoculum amount of Lactobacillus plantarum ST.
[0039] 2. Addition of sucrose: Cool the fermentation base solution to 30°C-40°C, divide it randomly into 6 experimental groups with equal volumes, and divide them into 5%, 6%, 7%, 8%, 9%, 10% (mass volume ratio) after adding sucrose, inoculate Lactobacillus plantarum ST according to the optimal inoculum amount, and ferment at 37°C for 20 hours. After...
Embodiment 3
[0044] Example 3 The functionality of cloud ginseng enzyme
[0045] After fermentation, the titratable acidity and protease activity of cloud ginseng enzyme were detected according to GB / T 12456-2008 and SB / T 10317-1999 respectively. According to DPPH (1,1-diphenyl-2-trinitrophenylhydrazine ) free radical scavenging rate test to evaluate the in vitro antioxidant activity of ginseng enzyme. The results are shown in Table 2. Compared with before fermentation, the organic acid content, protease activity, and DPPH free radical scavenging ability in the cloud ginseng enzyme increased by 17.42% respectively ( P P P <0.05), the biological function was significantly increased.
[0046] Table 4 Criteria for Sensory Evaluation of Yunshen Enzyme
[0047]
[0048] Table 5 Yun ginseng enzyme functional index
[0049]
[0050] Note: Different letters in the same column indicate significant differences ( P <0.05).
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