Fish skin collagen preparation method
A technology of fish skin collagen and fish skin, which is applied in the preparation method of peptides, chemical instruments and methods, animal/human protein, etc., can solve the problems of low extraction rate, high cost, pollution of the environment, etc., and achieve a wide range of uses
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[0020] The present invention provides a method for preparing fish skin collagen, which includes the following steps: (1) preparing fish dermis; (2) soaking fish dermis in acid solution II until it becomes a colloid, then putting it into a filter bag, and extruding the colloid under pressure , Obtain fish skin collagen colloid; (3) add alkaline substance solution to fish skin collagen colloid to flocculate and precipitate fish skin collagen; (4) purification treatment.
[0021] In the above preparation method, the step (1) describes how to process the collected fish skin raw materials into the directly processed object of the present invention, that is, fish dermis. In a specific embodiment, the step (1) is to soak the fish skin with acid solution I until the fish skin swells, remove the fish skin epidermis, and obtain fish dermis. The physical method of removing the epidermis is scraping, and the tools used can be all hard tools with friction and scraping functions, such as scrap...
Embodiment 1
[0033] Example 1. Preparation of Cod Leather
[0034] Weigh 100g of pollock fish skin into a glass beaker, put it in 1L of hydrochloric acid aqueous solution with a concentration of 0.1mol / L, soak for 2 hours, observe that the skin with more pigment is like tofu, use a steel brush to remove the pigment part White genuine leather. The photos of the leather obtained after the treatment of the whole pollock skin are as follows: figure 1 , figure 2 Shown. The main components of the whole skin, dermis and epidermis of cod skin were tested, and the results are shown in Table 1. It can be seen that the water content of the whole cod skin is 67.3%, and the collagen content in the dry matter of the whole skin is 47.3%; the cod skin is gray-black, the water content of the epidermis is 74.3%, and the collagen content in the dry matter of the epidermis is 36.7%, dark gray The water content of cod dermis is 63.4%, and the collagen content in the dry matter of the dermis is 56.2%, which is...
Embodiment 2
[0037] Example 2. Preparation of flounder dermal collagen
[0038] Weigh 100 kg of plaice fish skin, put it in 1500L of phosphoric acid aqueous solution with a concentration of 0.05mol / L, soak at 20°C for 2 hours and take it out. Remove the surface liquid of the fish skin on the filter. It is observed that the skin with more pigment is like tofu. Scrape off most of the gray-black epidermis with the back of the knife, and then use a steel brush to brush off the remaining epidermis to obtain a white dermis; then put it into 500L NaOH aqueous solution with a mass concentration of 0.01%, soak it at 20°C for 2 hours, and filter it. Then, use 0.05mol / L lactic acid aqueous solution to swell to 2-5 times the weight of the fish skin material, then put the swollen fish skin into a filter cloth bag, extrude the collagen colloid, and neutralize it with 0.5% NaOH solution to make the protein After flocculation and precipitation for 0.5 hours, the supernatant is filtered to remove the supernat...
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